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Brain targeted delivery of carmustine using solid lipid nanoparticles modified with tamoxifen and lactoferrin for antitumor proliferation
- Source :
- International Journal of Pharmaceutics. 499:10-19
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Solid lipid nanoparticles (SLNs) conjugated with tamoxifen (TX) and lactoferrin (Lf) were applied to carry anticancer carmustine (BCNU) across the blood-brain barrier (BBB) for enhanced antiproliferation against glioblastoma multiforme (GBM). BCNU-loaded SLNs with modified TX and Lf (TX-Lf-BCNU-SLNs) were used to penetrate a monolayer of human brain-microvascular endothelial cells (HBMECs) and human astrocytes and to target malignant U87MG cells. The surface TX and Lf on TX-Lf-BCNU-SLNs improved the characteristics of sustained release for BCNU. When compared with BCNU-loaded SLNs, TX-Lf-BCNU-SLNs increased the BBB permeability coefficient for BCNU about ten times. In addition, TX-BCNU-SLNs considerably promoted the fluorescent intensity of intracellular acetomethoxy derivative of calcein (calcein-AM) in HBMECs via endocytosis. However, the conjugated Lf could only slightly increase the fluorescence of calcein-AM. Moreover, the order of formulation in the inhibition to U87MG cells was TX-Lf-BCNU-SLNs>TX-BCNU-SLNs>Lf-BCNU-SLNs>BCNU-SLNs. TX-Lf-BCNU-SLNs can be effective in infiltrating the BBB and delivering BCNU to GBM for future chemotherapy application.
- Subjects :
- 0301 basic medicine
medicine.medical_treatment
Pharmaceutical Science
Antineoplastic Agents
Pharmacology
Endocytosis
Blood–brain barrier
Permeability
03 medical and health sciences
chemistry.chemical_compound
Drug Delivery Systems
0302 clinical medicine
Cell Line, Tumor
Solid lipid nanoparticle
medicine
Humans
Chemotherapy
Carmustine
biology
Lactoferrin
business.industry
Brain
Endothelial Cells
Fluoresceins
Lipids
body regions
Calcein
Tamoxifen
030104 developmental biology
medicine.anatomical_structure
chemistry
Blood-Brain Barrier
Astrocytes
Delayed-Action Preparations
biology.protein
Nanoparticles
Glioblastoma
business
030217 neurology & neurosurgery
Intracellular
medicine.drug
Subjects
Details
- ISSN :
- 03785173
- Volume :
- 499
- Database :
- OpenAIRE
- Journal :
- International Journal of Pharmaceutics
- Accession number :
- edsair.doi.dedup.....eb32cd62f03cfcf836ae1b5dfa8dcc83