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Rapid Dissociation of HIV-1 from Cultured Cells Severely Limits Infectivity Assays, Causes the Inactivation Ascribed to Entry Inhibitors, and Masks the Inherently High Level of Infectivity of Virions
- Source :
- Journal of Virology. 84:3106-3110
- Publication Year :
- 2010
- Publisher :
- American Society for Microbiology, 2010.
-
Abstract
- By using immunofluorescence microscopy to observe and analyze freshly made HIV-1 virions adsorbed onto cells, we found that they are inherently highly infectious, rather than predominantly defective as previously suggested. Surprisingly, polycations enhance titers 20- to 30-fold by stabilizing adsorption and preventing a previously undescribed process of rapid dissociation, strongly implying that infectivity assays for many viruses are limited not only by inefficient virus diffusion onto cells but also by a postattachment race between entry and dissociation. This kinetic competition underlies inhibitory effects of CCR5 antagonists and explains why adaptive HIV-1 mutations overcome many cell entry limitations by accelerating entry.
- Subjects :
- Immunology
Human immunodeficiency virus (HIV)
Immunofluorescence Microscopy
Biology
medicine.disease_cause
Microbiology
Dissociation (chemistry)
Virus
Virology
medicine
Humans
Cells, Cultured
Infectivity
Cell entry
Virion
Virus Internalization
biology.organism_classification
Virus-Cell Interactions
Cell biology
Titer
Insect Science
CCR5 Receptor Antagonists
Lentivirus
HIV-1
Virus Inactivation
Adsorption
HeLa Cells
Subjects
Details
- ISSN :
- 10985514 and 0022538X
- Volume :
- 84
- Database :
- OpenAIRE
- Journal :
- Journal of Virology
- Accession number :
- edsair.doi.dedup.....eb3a133d0dcf1f2d6fac418f2f5c8959