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Inhibition of miR-1298-5p attenuates sepsis lung injury by targeting SOCS6
- Source :
- Molecular and Cellular Biochemistry. 476:3745-3756
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Sepsis is one of the leading causes of morbidity and mortality and a major cause of acute lung injury (ALI). carried by exosomes play a role in a variety of diseases. However,there are not many studies of exosomal miRNAs in sepsis and sepsis lung injury.miR-1298-5p and suppressor of cytokine signaling 6 (SOCS6) were silenced or overexpressed in human bronchial epithelial cells (BEAS-2B). PKH-67 Dye was used to trace exosome endocytosis. Cell permeability was evaluated by measuring trans-epithelial electrical resistance (TEER) and FITC dextran flux. ELISA kits were used for cytokine detection. Quantitative RT-PCR and western blots were used to evaluate gene expression. miR-1298-5p was elevated in exosomes from patients with sepsis lung injury (Sepsis_exo). Treatment of BEAS-2B cells using Sepsis_exo significantly inhibited cell proliferation, and induced cell permeability and inflammatory response. miR-1298-5p directly targeted SOCS6. Overexpressing SOCS6 reversed miR-1298-5p-induced cell permeability and inflammatory response. Inhibition of STAT3 blocked SOCS6-silencing caused significant increase of cell permeability and inflammation. Exosomes isolated from patients of sepsis lung injury increased cell permeability and inflammatory response in BEAS-2B cells through exosomal miR-1298-5p which targeted SOCS6 via STAT3 pathway. The findings highlight the importance of miR-1298-5p/SOCS6/STAT3 axis in sepsis lung injury and provide new insights into therapeutic strategies for sepsis lung injury.
- Subjects :
- STAT3 Transcription Factor
0301 basic medicine
medicine.medical_treatment
Acute Lung Injury
Clinical Biochemistry
Suppressor of Cytokine Signaling Proteins
Inflammation
Lung injury
Exosomes
Exosome
Exocytosis
Cell Line
Sepsis
03 medical and health sciences
0302 clinical medicine
medicine
Humans
SOCS6
Molecular Biology
Lung
Cell growth
business.industry
Cell Biology
General Medicine
medicine.disease
MicroRNAs
030104 developmental biology
Cytokine
medicine.anatomical_structure
030220 oncology & carcinogenesis
Cancer research
medicine.symptom
business
Signal Transduction
Subjects
Details
- ISSN :
- 15734919 and 03008177
- Volume :
- 476
- Database :
- OpenAIRE
- Journal :
- Molecular and Cellular Biochemistry
- Accession number :
- edsair.doi.dedup.....eb52fb5b2709f3d88551360ecb7e1003