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Population Genetic Structure of QoI-Resistant Pestalotiopsis longiseta Isolates Causing Tea Gray Blight
- Source :
- SC10201707040009
- Publication Year :
- 2016
- Publisher :
- Scientific Societies, 2016.
-
Abstract
- Resistance to the quinone outside inhibitor (QoI) fungicides in the tea gray blight-causing fungus Pestalotiopsis longiseta is a serious problem in Japanese tea cultivation. We conducted a population genetic analysis of QoI-resistant P. longiseta isolates on the Makinohara Plateau, Shizuoka Prefecture, Japan’s largest tea-growing area, to elucidate the disease’s epidemiology and the spread of QoI resistance. Inter simple sequence repeat (ISSR) analysis of 1,083 isolates from 395 fields collected from 2009 to 2012 detected 42 ISSR types, designated as PL01 to PL42. A total of 18, seven, and 38 ISSR types were detected in highly resistant, moderately resistant, and sensitive isolates, respectively. No distinct phylogenetic relationship corresponding to QoI sensitivity or sampling location was observed. No annual changes in the population genetic structure of highly resistant isolates were observed during the study period. A different ISSR type was predominant among QoI-resistant isolates in each region. Analysis of molecular variance revealed significant genetic differentiation in populations of highly resistant isolates among regions (FCT = 0.213) and farmers (FCT = 0.071). Consequently, we speculate that QoI-resistant P. longiseta strains occurred in a number of clonal lineages and spread by both natural and artificial transmission, such as rain splash and plucking machines, throughout each region on the Makinohara Plateau.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Veterinary medicine
education.field_of_study
biology
Population
Plant Science
Fungus
biology.organism_classification
01 natural sciences
Analysis of molecular variance
Genetic analysis
Fungicide
03 medical and health sciences
030104 developmental biology
Genetic structure
Botany
Blight
Pestalotiopsis longiseta
education
Agronomy and Crop Science
010606 plant biology & botany
Subjects
Details
- ISSN :
- 19437692 and 01912917
- Volume :
- 100
- Database :
- OpenAIRE
- Journal :
- Plant Disease
- Accession number :
- edsair.doi.dedup.....eb5c8f0985e699d338d05d657969f4c7
- Full Text :
- https://doi.org/10.1094/pdis-09-15-1114-re