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Enhancement of ganoderic acid production by promoting sporulation in a liquid static culture of Ganoderma species
- Source :
- Journal of biotechnology. 328
- Publication Year :
- 2020
-
Abstract
- Ganoderic acids (GAs) produced by Ganoderma are a type of lanostane-type triterpenoids with anticancer and antimetastatic activities; however, low production of GAs limits its wide application. In this study, a novel strategy by promoting sporulation of Ganoderma was developed to increase GA production. First, a high-spore producing Ganoderma strain G. 260125 was obtained from dikaryotic strain CGMCC 5.0026, and the sporulation-specific gene of this strain exhibits a higher transcription level than CGMCC 5.0026. Then, the effect of promoting sporulation on GA content was investigated. The maximum ganoderic acid (GA)-T, GA-Mk, and GA-Me contents in G. 260125 in shake flasks were 358.97, 78.32, and 12.75 μg/100 mg dry weight, respectively, which were 3.42, 2.91, and 1.73 times higher than those obtained in CGMCC 5.0026. Moreover, total and individual GA contents in spores were significantly higher than those in liquid static culture. Both concentrations of intermediates and transcription levels of GA biosynthetic genes also improved in G. 260125 during fermentation compared with those in CGMCC 5.0026. For scaling-up experiments, GA-T, GA-Me, and GA-Mk production in G. 260125 improved by 2.2-, 2.6-, and 2.1-fold compared with those in CGMCC 5.0026. In addition, the effectiveness of the developed strategy was also confirmed in three different Ganoderma strains. This work illustrated that promoting sporulation efficiently improves GA production in liquid static cultures of Ganoderma.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Reishi
Ganoderma
Bioengineering
01 natural sciences
Applied Microbiology and Biotechnology
03 medical and health sciences
chemistry.chemical_compound
Dry weight
010608 biotechnology
Food science
Dikaryon
biology
Strain (chemistry)
Chemistry
Ganoderic acid
General Medicine
biology.organism_classification
Triterpenes
Spore
030104 developmental biology
Fermentation
Biotechnology
Biosynthetic genes
Subjects
Details
- ISSN :
- 18734863
- Volume :
- 328
- Database :
- OpenAIRE
- Journal :
- Journal of biotechnology
- Accession number :
- edsair.doi.dedup.....eb913e8f600640239f9e268329144ca0