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The C-S lysis of L-cysteine conjugates by aspartate and alanine aminotransferase enzymes
- Source :
- Humanexperimental toxicology. 14(5)
- Publication Year :
- 1995
-
Abstract
- One biotransformation pathway which is responsible for the generation of mutagenic and cytotoxic metabolites is that of the C-S lysis (CSL) of L-cysteine conjugates. Thirteen cysteine S-conjugates, synthesised in our labora tories, were incubated with porcine heart aspartate aminotransferase (ASAT) and alanine aminotransferase (ALAT), and the C-S lyase activity for each enzyme-sub strate combination was determined. ASAT and ALAT were shown to exhibit CSL activity. It was also demonstrated that this activity was inhibited in the presence of the pyri doxal phosphate (PLP)-dependent enzyme inhibitor amino(oxyacetic acid) (AOAA) confirming the pyridoxal phosphate dependent mechanism by which C-S lysis is known to take place. Since the activities of these enzymes are used as biomarkers for the assessment of organ dam age, the potential interaction of L-cysteine conjugates with them may suppress their activity through direct inhibition.
- Subjects :
- 0301 basic medicine
Lysis
Swine
Health, Toxicology and Mutagenesis
Aspartate transaminase
Toxicology
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Animals
Aspartate Aminotransferases
Cysteine
Pyridoxal phosphate
Lyase activity
Biotransformation
chemistry.chemical_classification
030102 biochemistry & molecular biology
biology
Myocardium
Alanine Transaminase
General Medicine
Enzyme
chemistry
Alanine transaminase
Biochemistry
Enzyme inhibitor
030220 oncology & carcinogenesis
biology.protein
Subjects
Details
- ISSN :
- 09603271
- Volume :
- 14
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Humanexperimental toxicology
- Accession number :
- edsair.doi.dedup.....eba4f9c22e88af877f8acc8ecea38be6