Rapid quantitative horizontal paper chromatography at an elevated temperature: determination of aspartic and glutamic acids

BY the use of rectangular strips of filter paper, rather than filter paper disks, in combination with a horizontal solvent flow at an elevated temperature, amino-acids are rapidly separated as round compact spots rather than circular bands1. This is desirable since such spots in many cases lend themselves to quantitative study by the maximum colour-density technique. Recently, such a procedure has been applied to the analysis of tryptophan in an alkaline hydrolysate of α-casein2.