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Fatty acid remodeling in cellular glycerophospholipids following the activation of human T cells

Authors :
Marc E. Surette
Philippe Pierre Robichaud
Jean Eric Munganyiki
Katherine Boulay
Source :
Journal of Lipid Research, Vol 54, Iss 10, Pp 2665-2677 (2013)
Publication Year :
2013

Abstract

Changes in fatty acid (FA) and glycerophospholipid (GPL) metabolism associated with cell cycle entry are not fully understood. In this study FA-GPL remodeling was investigated in resting and proliferating primary human T cells. Significant changes were measured in the composition and distribution of FAs in GPLs following receptor activation of human T cells. The FA distribution of proliferating T cells was very similar to that of the human Jurkat T cell line and when the stimulus was removed from proliferating T cells, they stopped proliferating and the FA distribution largely reverted back to that of resting T cells. The cellular content of saturated and monounsaturated FAs was significantly increased in proliferating cells, which was associated with an induction of FA synthase and stearoyl-CoA desaturase-1 gene expression. Additionally, cellular arachidonate was redistributed in GPLs in a distinct pattern that was unlike any other FAs. This redistribution was associated with an induction of CoA-dependent and CoA-independent remodeling. Accordingly, significant changes in the expression of several acyl-CoA synthetases, lysophospholipid acyltransferases, and phospholipase A2 were measured. Overall, these results suggest that metabolic pathways are activated in proliferating T cells that may represent fundamental changes associated with human cell proliferation.

Details

ISSN :
15397262
Volume :
54
Issue :
10
Database :
OpenAIRE
Journal :
Journal of lipid research
Accession number :
edsair.doi.dedup.....ebd1897e1ffd44edd65850a140d5f369