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Fatty acid remodeling in cellular glycerophospholipids following the activation of human T cells
- Source :
- Journal of Lipid Research, Vol 54, Iss 10, Pp 2665-2677 (2013)
- Publication Year :
- 2013
-
Abstract
- Changes in fatty acid (FA) and glycerophospholipid (GPL) metabolism associated with cell cycle entry are not fully understood. In this study FA-GPL remodeling was investigated in resting and proliferating primary human T cells. Significant changes were measured in the composition and distribution of FAs in GPLs following receptor activation of human T cells. The FA distribution of proliferating T cells was very similar to that of the human Jurkat T cell line and when the stimulus was removed from proliferating T cells, they stopped proliferating and the FA distribution largely reverted back to that of resting T cells. The cellular content of saturated and monounsaturated FAs was significantly increased in proliferating cells, which was associated with an induction of FA synthase and stearoyl-CoA desaturase-1 gene expression. Additionally, cellular arachidonate was redistributed in GPLs in a distinct pattern that was unlike any other FAs. This redistribution was associated with an induction of CoA-dependent and CoA-independent remodeling. Accordingly, significant changes in the expression of several acyl-CoA synthetases, lysophospholipid acyltransferases, and phospholipase A2 were measured. Overall, these results suggest that metabolic pathways are activated in proliferating T cells that may represent fundamental changes associated with human cell proliferation.
- Subjects :
- T cell
T-Lymphocytes
stearoyl-CoA desaturase 1
lysophospholipid acyltransferase
Gene Expression
QD415-436
Arachidonic Acids
Glycerophospholipids
Biology
Lymphocyte Activation
Jurkat cells
Biochemistry
chemistry.chemical_compound
acyl-CoA synthetase
Endocrinology
Phospholipase A2
medicine
arachidonic acid
Humans
Cells, Cultured
Research Articles
fatty acid synthase
Fatty Acids
Cell Biology
Cell cycle
Cell biology
Fatty Acid Synthase, Type I
Fatty acid synthase
medicine.anatomical_structure
chemistry
Glycerophospholipid
biology.protein
Arachidonic acid
phospholipase A2
Stearoyl-CoA desaturase-1
Stearoyl-CoA Desaturase
Subjects
Details
- ISSN :
- 15397262
- Volume :
- 54
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Journal of lipid research
- Accession number :
- edsair.doi.dedup.....ebd1897e1ffd44edd65850a140d5f369