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Molecular cloning, expression analysis and functional characterization of interleukin-22 in So-iny mullet, Liza haematocheila
- Source :
- Molecular Immunology. 63:245-252
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- In the present study, interleukin-22 (IL-22) from So-iny mullet (Liza haematocheila) was identified, and its tissue expression in both healthy and Streptococcus dysgalactiae-infected fish was examined. The full length cDNA sequence of mullet IL-22 was 1070bp, containing an open reading frame of 555bp. The deduced amino acid sequence shared high similarity (45.1-67.9%) with IL-22 from other fish species. Mullet IL-22 also contained an IL-10 family signature and four cysteine residues that were well conserved in other vertebrate IL-22 molecules. Mullet IL-22 mRNA was highly expressed in kidney, moderately expressed in liver and gut, and relatively weakly expressed in spleen, and its expression was significantly up-regulated in all the examined tissues following S. dysgalactiae infection. Furthermore, recombinant mullet IL-22 protein was shown to promote the expression of β-defensin in the four tissues and to increase the survival rate of the fish infected with S. dysgalactiae. Our results suggest mullet IL-22 plays an important role in the immune defense against bacterial infection and has the potential to be used to treat bacterial diseases in fish.
- Subjects :
- DNA, Complementary
beta-Defensins
Molecular Sequence Data
Immunology
Molecular cloning
Mullet
law.invention
law
Streptococcal Infections
Complementary DNA
Gene expression
Animals
Amino Acid Sequence
RNA, Messenger
Cloning, Molecular
Molecular Biology
Peptide sequence
Phylogeny
Messenger RNA
Base Sequence
Sequence Homology, Amino Acid
biology
Gene Expression Profiling
Interleukins
Streptococcus
biology.organism_classification
Survival Analysis
Molecular biology
Recombinant Proteins
Smegmamorpha
Open reading frame
Recombinant DNA
Subjects
Details
- ISSN :
- 01615890
- Volume :
- 63
- Database :
- OpenAIRE
- Journal :
- Molecular Immunology
- Accession number :
- edsair.doi.dedup.....ec4c1249724367808ba6c0cc78b79689