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Renewable fatty acid ester production in Clostridium
- Source :
- Nature Communications, Nature Communications 12: 4368 2021, Repositorio UNA, Universidad Nacional de Costa Rica, instacron:UNA, Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
- Publication Year :
- 2021
-
Abstract
- Bioproduction of renewable chemicals is considered as an urgent solution for fossil energy crisis. However, despite tremendous efforts, it is still challenging to generate microbial strains that can produce target biochemical to high levels. Here, we report an example of biosynthesis of high-value and easy-recoverable derivatives built upon natural microbial pathways, leading to improvement in bioproduction efficiency. By leveraging pathways in solventogenic clostridia for co-producing acyl-CoAs, acids and alcohols as precursors, through rational screening for host strains and enzymes, systematic metabolic engineering-including elimination of putative prophages, we develop strains that can produce 20.3 g/L butyl acetate and 1.6 g/L butyl butyrate. Techno-economic analysis results suggest the economic competitiveness of our developed bioprocess. Our principles of selecting the most appropriate host for specific bioproduction and engineering microbial chassis to produce high-value and easy-separable end products may be applicable to other bioprocesses.<br />Esters can be used as fuels and specialty chemicals for food flavoring, cosmetic and pharmaceutical industries. Here, the authors systematically engineer clostridia, including discovery and deletion of prophages to increase strain stability, for the production of butyl acetate and butyl butyrate from corn stover at low cost.
- Subjects :
- 0106 biological sciences
Science
General Physics and Astronomy
CLOSTRIDIUM
Acetates
01 natural sciences
Article
General Biochemistry, Genetics and Molecular Biology
Applied microbiology
Clostridia
03 medical and health sciences
chemistry.chemical_compound
Clostridium
Acetyl Coenzyme A
010608 biotechnology
ACID ESTER PRODUCTION
Biomass
Food science
Bioprocess
BIOPROCESOS
030304 developmental biology
0303 health sciences
Multidisciplinary
biology
Fatty Acids
Proteins
food and beverages
Esters
General Chemistry
equipment and supplies
NAD
biology.organism_classification
Bioproduction
Recombinant Proteins
ENERGÍA
Butyrates
Corn stover
Metabolic Engineering
chemistry
QUÍMICA DEL MEDIO AMBIENTE
Biofuels
Fermentation
BIOQUÍMICA
Butyl acetate
Metabolic Networks and Pathways
Butyl butyrate
Speciality chemicals
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Nature Communications, Nature Communications 12: 4368 2021, Repositorio UNA, Universidad Nacional de Costa Rica, instacron:UNA, Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
- Accession number :
- edsair.doi.dedup.....ec4f460cc829635d9a83878253c71871