Adenine-functionalized polypropylene glycol: A novel stationary phase for gas chromatography offering good inertness for acids and bases combined with a unique selectivity

This work presents the investigation of utilizing adenine-functionalized polypropylene glycol (APPG) for capillary gas chromatographic separations. The statically coated APPG column (0.25 mm i.d.) showed moderate polarity and high column efficiencies of 4660 plates/m and 4376 plates/m determined by n-octanol and naphthalene, respectively. Remarkably, the APPG column baseline resolved all the components of the Grob test mixture and displayed good peak shapes for some stringent analytes that are prone to peak tailing or severe adsorption. Also, it achieved complete separation of dimethylaniline isomers, which are difficult to be resolved due to their high resemblance in structures and properties. The above results demonstrate the high selectivity and inertness of the APPG column and its distinct advantages over the polypropylene glycol (PPG) column and commercial polyethylene glycol (PEG) column. In addition, its separation performance has good repeatability with the RSD values on retention times below 0.05% for run-to-run, 0.11–0.12% for day-to-day and 1.7–1.9% for column-to-column, respectively. Further, the APPG column was applied to determination of isomer impurities in commercial dimethylaniline products and to determination of the additives of anilines and phenols in a hair-dye product, proving its great potential for practical GC analysis.