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The effect of zymosan and the protective effect of various antioxidants on fracture healing in rats
- Source :
- Archives of Orthopaedic and Trauma Surgery. 127:493-501
- Publication Year :
- 2007
- Publisher :
- Springer Science and Business Media LLC, 2007.
-
Abstract
- To investigate the effects of free oxygen radicals and various antioxidants on bone healing after experimental formation of fracture. Fifty male rats were used and divided into five groups (ten rats in each). The right forelimbs of the rats were broken by bimanual compression method. One hour before this procedure, 5 ml/kg of intraperitoneal (i.p.) physiologic saline were given to the control Group 1. All 40 rats in the experimental Groups 2, 3, 4 and 5 were treated with i.p. zymosan at a dosage of 100 mg/kg to induce the production of free radicals by stimulating NADPH oxidase in polymorphonuclear leukocytes. Zymosan induction was stopped on the fifth post-fracture day. In addition to the zymosan, i.p. 1 g/kg/day of dimethyl sulfoxide were given to the animals in Group 3, 50 mg/kg/d of Ginko biloba Extract (EGb 761) in Group 4 and 500 mg/kg/day of vitamin C in Group 5. Radiographs of the fractures of all animals were obtained to assess callus formation, remodeling and bridging bone formation under ether anesthetics on postfracture day 7, 14 and 21. All rats were euthanized on day 22, and sections of the radius and ulna were examined both histologically with light and electron microscopy and ultrastructurally. Statistical analysis was made with Kruskal-Wallis variance analyze test and comparison between groups was performed by Dunn’s multiple comparision test. An impairment of bone healing was observed in Group 2 inducted with purely zymosan. Variable results were obtained for bone healing in the groups treated with various antioxidants. There was very significant difference of fracture healing between Groups 1 and 2 both histologically and radiologically (P
- Subjects :
- Male
medicine.medical_specialty
Time Factors
Antioxidant
Free Radicals
Callus formation
medicine.medical_treatment
Ascorbic Acid
Bone healing
Antioxidants
Statistics, Nonparametric
Random Allocation
chemistry.chemical_compound
Internal medicine
Animals
Medicine
Dimethyl Sulfoxide
Orthopedics and Sports Medicine
Rats, Wistar
Saline
Fracture Healing
NADPH oxidase
biology
Vitamin C
Plant Extracts
business.industry
Dimethyl sulfoxide
Zymosan
Ginkgo biloba
Free Radical Scavengers
General Medicine
Ulna Fractures
Rats
Surgery
Disease Models, Animal
Endocrinology
chemistry
Research Design
biology.protein
Radius Fractures
business
Subjects
Details
- ISSN :
- 14343916 and 09368051
- Volume :
- 127
- Database :
- OpenAIRE
- Journal :
- Archives of Orthopaedic and Trauma Surgery
- Accession number :
- edsair.doi.dedup.....ec79df6e48351c6ce348c4b1df0b9fbc