Back to Search
Start Over
Egg yolk oils exert anti-inflammatory effect via regulating Nrf2/NF-κB pathway
- Source :
- Journal of Ethnopharmacology. 274:114070
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Ethnopharmacological relevance Egg yolk oils (EYO) is a traditional Chinese medicine obtained from Gallus gallus domesticus Brisson, which has been used to treat inflammatory related diseases such as cheilitis, ulceration and acute anal fissure. However, the detailed anti-inflammatory mechanism of EYO is still unknown. Aim of the study The anti-inflammatory activity and mechanism of EYO were investigated in tumor necrosis factor (TNF)-α induced Caco-2 cells. Materials and methods EYO was obtained by direct-heat extraction (HE), ethanol extraction (EE) and petroleum ether extraction (PE), respectively. Fatty acid compositions of three EYO were measured by gas chromatography (GC). Cell viability, enzyme-linked immunosorbent assay (ELISA), transcriptome, RT-PCR and Western blotting were also performed. Results Fatty acid compositions of three EYO were different with varied extraction methods. EYO significantly reduced interleukin (IL)-8 secretion. EYO exerted anti-inflammatory effect via coordinating regulation of Nrf2/NF-κB pathways based on the results of transcriptome, Q-PCR and Western blotting. In detail, PE and HE inhibited the NF-κB pathway, whereas EE exerted anti-inflammatory activity via the Nrf2/NF-κB pathways. Conclusions The aforementioned results showed the anti-inflammatory mechanism of EYO. These findings might be beneficial to clinical applications of EYO.
- Subjects :
- Thioredoxin Reductase 1
Cell Survival
NF-E2-Related Factor 2
medicine.drug_class
Glutamate-Cysteine Ligase
Anti-Inflammatory Agents
Pharmacology
Anti-inflammatory
Transcriptome
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
NF-KappaB Inhibitor alpha
Drug Discovery
NAD(P)H Dehydrogenase (Quinone)
medicine
Humans
Viability assay
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
Chemistry
Fatty Acids
NF-kappa B
Interleukin
Fatty acid
NF-κB
Egg Yolk
Blot
030220 oncology & carcinogenesis
Cytokines
Tumor necrosis factor alpha
Caco-2 Cells
Oils
Heme Oxygenase-1
Signal Transduction
Subjects
Details
- ISSN :
- 03788741
- Volume :
- 274
- Database :
- OpenAIRE
- Journal :
- Journal of Ethnopharmacology
- Accession number :
- edsair.doi.dedup.....ec8099774773a7ff4a13caa749eb1a7d