Back to Search
Start Over
Effects of gut microbiota manipulation on ex vivo lipolysis in human abdominal subcutaneous adipocytes
- Source :
- Adipocyte, 7(2), 106-112. Routledge/Taylor & Francis Group, Adipocyte 7 (2018) 2, Adipocyte, 7(2), 106-112
- Publication Year :
- 2018
-
Abstract
- The intestinal microbiota may contribute to the development of obesity by affecting host lipid metabolism and insulin sensitivity. To investigate the effects of microbiota manipulation on ex vivo basal and beta-adrenergically-stimulated lipolysis in human adipocytes, 36 obese men were randomized to amoxicillin (broad-spectrum antibiotic), vancomycin (narrow-spectrum antibiotic) or placebo treatment (7 d, 1500 mg/d). Before and after treatment, ex vivo adipose tissue lipolysis was assessed under basal conditions and during stimulation with the non-selective beta-agonist isoprenaline using freshly isolated mature adipocytes. Gene (targeted microarray) and protein expression were analyzed to investigate underlying pathways. Antibiotics treatment did not significantly affect basal and maximal isoprenaline-mediated glycerol release from adipocytes. Adipose tissue beta-adrenoceptor expression or post-receptor signalling was also not different between groups. In conclusion, 7 d oral antibiotics treatment has no effect on ex vivo lipolysis in mature adipocytes derived from adipose tissue of obese insulin resistant men.
- Subjects :
- Adult
Male
EXPRESSION
medicine.medical_specialty
DIET-INDUCED OBESITY
Histology
Lipolysis
Subcutaneous Fat
Adipose tissue
HORMONE-SENSITIVE LIPASE
PROTEIN
Hormone-sensitive lipase
Gut flora
chemistry.chemical_compound
Insulin resistance
Internal medicine
Adipocytes
medicine
ACETATE
Humans
Obesity
PHOSPHORYLATION
Sensory Science and Eating Behaviour
VLAG
Aged
Fatty acid metabolism
biology
business.industry
Microbiota
FREE FATTY-ACIDS
Cell Biology
Middle Aged
biology.organism_classification
medicine.disease
Gastrointestinal Microbiome
Sensoriek en eetgedrag
Endocrinology
DIFFERENTIATION
chemistry
Adipose Tissue
Adipose triglyceride lipase
PROPIONIC-ACID
business
ADIPOSE TRIGLYCERIDE LIPASE
Ex vivo
Subjects
Details
- Language :
- English
- ISSN :
- 21623945
- Database :
- OpenAIRE
- Journal :
- Adipocyte, 7(2), 106-112. Routledge/Taylor & Francis Group, Adipocyte 7 (2018) 2, Adipocyte, 7(2), 106-112
- Accession number :
- edsair.doi.dedup.....eca6c6f9d2b3fda53db5855dea4547c0