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Discovery and Validation of a Molecular Signature for the Noninvasive Diagnosis of Human Renal Allograft Fibrosis
- Source :
- Transplantation. 93:1136-1146
- Publication Year :
- 2012
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2012.
-
Abstract
- BACKGROUND Tubulointerstitial fibrosis (fibrosis), a histologic feature associated with a failing kidney allograft, is diagnosed using the invasive allograft biopsy. A noninvasive diagnostic test for fibrosis may help improve allograft outcome. METHODS We obtained 114 urine specimens from 114 renal allograft recipients: 48 from 48 recipients with fibrosis in their biopsy results and 66 from 66 recipients with normal biopsy results. Levels of messenger RNAs (mRNAs) in urinary cells were measured using kinetic, quantitative polymerase chain reaction assays, and the levels were related to allograft diagnosis. A discovery set of 76 recipients (32 with allograft fibrosis and 44 with normal biopsy results) was used to develop a diagnostic signature, and an independent validation set of 38 recipients (16 with allograft fibrosis and 22 with normal biopsy results) was used to validate the signature. RESULTS In the discovery set, urinary cell levels of the following mRNAs were significantly associated with the presence of allograft fibrosis: vimentin (P
- Subjects :
- Adult
Male
Pathology
medicine.medical_specialty
Sodium-Potassium-Chloride Symporters
Biopsy
Urinary system
Biology
Kidney
Polymerase Chain Reaction
Sensitivity and Specificity
Article
chemistry.chemical_compound
Postoperative Complications
Fibrosis
RNA, Ribosomal, 18S
medicine
Humans
Vimentin
RNA, Messenger
Solute Carrier Family 12, Member 1
Transplantation
medicine.diagnostic_test
Kidney metabolism
Middle Aged
Cadherins
medicine.disease
Kidney Transplantation
Cross-Sectional Studies
Logistic Models
medicine.anatomical_structure
Real-time polymerase chain reaction
ROC Curve
chemistry
Plasminogen activator inhibitor-1
Tubulointerstitial fibrosis
Female
Kidney Diseases
Biomarkers
Subjects
Details
- ISSN :
- 00411337
- Volume :
- 93
- Database :
- OpenAIRE
- Journal :
- Transplantation
- Accession number :
- edsair.doi.dedup.....ecb075ce57d574e7c5e831f93d62d677