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Moxifloxacin: Clinically compatible contrast agent for multiphoton imaging

Authors :
Calvin J. Yoon
Yong Song Gho
Sekyu Hwang
Bumju Kim
Peter T. C. So
Yeoreum Yoon
Jun Ho Lee
Sungjee Kim
Gilgu Lee
Taejun Wang
Myoung Joon Kim
Viet-Hoan Le
Seunghun Lee
Myoung Ho Jang
Ki Hean Kim
Won Hyuk Jang
Seoyeon Bok
G-One Ahn
Jaewook Lee
Euiheon Chung
Seung-Jae Myung
Chun-Pyo Hong
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
So, Peter T. C.
Source :
Scientific Reports, SCIENTIFIC REPORTS(6)
Publication Year :
2016
Publisher :
Nature Publishing Group, 2016.

Abstract

Multiphoton microscopy (MPM) is a nonlinear fluorescence microscopic technique widely used for cellular imaging of thick tissues and live animals in biological studies. However, MPM application to human tissues is limited by weak endogenous fluorescence in tissue and cytotoxicity of exogenous probes. Herein, we describe the applications of moxifloxacin, an FDA-approved antibiotic, as a cell-labeling agent for MPM. Moxifloxacin has bright intrinsic multiphoton fluorescence, good tissue penetration and high intracellular concentration. MPM with moxifloxacin was demonstrated in various cell lines, and animal tissues of cornea, skin, small intestine and bladder. Clinical application is promising since imaging based on moxifloxacin labeling could be 10 times faster than imaging based on endogenous fluorescence.<br />David H. Koch Institute for Integrative Cancer Research at MIT (Bridge Initiative)

Details

Language :
English
ISSN :
20452322
Database :
OpenAIRE
Journal :
Scientific Reports
Accession number :
edsair.doi.dedup.....ecb5066c261590f72e0cd985320faed5
Full Text :
https://doi.org/10.1038/srep27142