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Neonatal exposure to genistein ameliorates high-fat diet-induced non-alcoholic steatohepatitis in rats
- Source :
- The British journal of nutrition. 106(1)
- Publication Year :
- 2011
-
Abstract
- Non-alcoholic steatohepatitis (NASH) is becoming a prevalent disease in developing countries with no effective therapy. Isoflavones such as genistein have been shown to prevent NASH in a rat model, but the effects of neonatal exposure to genistein on lipid metabolism have been rarely studied. In the present study, three doses of genistein (30, 300 or 1200 μg/rat per d) were injected (subcutaneously) into neonatal male Sprague–Dawley rats at postnatal days 1–5. After weaning, these rats were allowed free access to a high-fat diet for 6 weeks. The results demonstrate that NASH was induced by high fat feeding in the control rats, whereas genistein-treated rats displayed smaller body weight, and lower hepatic inflammation and steatosis. The mid dose of genistein was most effective. Neonatal exposure to genistein also resulted in a lower incidence of apoptotic cells in the liver. Additionally, neonatal genistein-treated rats showed lower hepatic expression of fatty acid synthase and sterol regulatory element-binding protein-1, but higher expression of PPARα, indicative of lower rates of lipid synthesis and higher rates of β-oxidation. These results indicate that neonatal treatment with genistein has a prolonged effect on hepatic lipid metabolism that is maintained post-weaning, offering a potential approach for the prevention of hepatic steatosis and NASH.
- Subjects :
- Male
medicine.medical_specialty
Time Factors
Medicine (miscellaneous)
Genistein
Apoptosis
Rats, Sprague-Dawley
chemistry.chemical_compound
Internal medicine
medicine
Weaning
Animals
Nutrition and Dietetics
biology
business.industry
Body Weight
Lipid metabolism
Organ Size
Isoflavones
medicine.disease
Dietary Fats
Rats
Fatty Liver
Fatty acid synthase
Endocrinology
chemistry
Animals, Newborn
Gene Expression Regulation
Liver
Dietary Supplements
biology.protein
Hepatocytes
Female
Steatohepatitis
Steatosis
business
Subjects
Details
- ISSN :
- 14752662
- Volume :
- 106
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- The British journal of nutrition
- Accession number :
- edsair.doi.dedup.....eccca25420a4a2470c2f4673cb362471