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Genetic diversity and molecular epidemiology of respiratory syncytial virus circulated in Antananarivo, Madagascar, from 2011 to 2017: Predominance of ON1 and BA9 genotypes
- Source :
- Journal of Clinical Virology, Journal of Clinical Virology, Elsevier, 2020, 129, pp.104506. ⟨10.1016/j.jcv.2020.104506⟩
- Publication Year :
- 2019
-
Abstract
- International audience; Background: Respiratory syncytial virus is the main cause of acute respiratory infections leading to a considerable morbidity and mortality among under-5 years children. A comprehensive scheme of RSV virus evolution is of great value in implementing effective universal RSV vaccine. Objective: We investigated the clinical spectrum and molecular characteristics of detected RSV over a period of seven years (January 2011 to June 2017) in Antananarivo, the capital city of Madagascar. Study design: 671 nasopharyngeal samples taken from children aged less than 5 years suffered from ARI were screened for RSV by real-time PCR. Clinical data were retrieved from case report forms. Genotype identification was performed by reverse-transcription PCR and sequencing of the second hyper variable region (HVR2) of the G glycoprotein. Results: Amongst samples tested, 292 (43.5 %) were found positive for RSV. RSV A predominated during the study period which accounted for 62.3 % (182/292) of positive samples while RSV B represented 37.0 % (108/ 292). Phylogenetic analyses identified NA1 and ON1 genotypes among RSV A. Though NA1 widespread from 2011 to 2013, ON1 became prevalent during the following years. Among RSV B, THB, CB1 and BA9 genotypes were detected. A co-circulation of THB and CB1 strains occurred during the 2011 season that was substituted by the BA9 from 2012. Malagasy ON1 strains carried some characteristic amino acid substitutions that distinguish them from the worldwide ON1 strains. By analyzing clinical spectrum, ON1 and BA genotypes seemed to prevail in mild infections compared to NA1. Conclusion: Results obtained here will have its implication in predicting temporal evolution of RSV at the local level. Considering the insularity of the country, information obtained should help in comparative analysis with global RSV strains to optimize vaccine efficacy.
- Subjects :
- 0301 basic medicine
Genotype
030106 microbiology
Clinical spectrum
Respiratory Syncytial Virus Infections
Biology
Respiratory syncytial virus
Virus
Genetic diversity
03 medical and health sciences
0302 clinical medicine
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Virology
Madagascar
Humans
030212 general & internal medicine
Respiratory system
Child
Phylogeny
[SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases
Molecular Epidemiology
Molecular epidemiology
Phylogenetic tree
Genetic Variation
Infant
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology
Vaccine efficacy
3. Good health
Infectious Diseases
Acute respiratory infection
Viral evolution
Respiratory Syncytial Virus, Human
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
Antananarivo
Subjects
Details
- ISSN :
- 18735967 and 13866532
- Volume :
- 129
- Database :
- OpenAIRE
- Journal :
- Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
- Accession number :
- edsair.doi.dedup.....ecd16ebed4ba0ec674c0eed0678252f4