Decreased CD3+CD16+ natural killer-like T-cell percentage and zeta-chain expression accompany chronic inflammation in haemodialysis patients

SUMMARY Aim: Clinical and experimental data indicate a deficient immune response in haemodialysis (HD) patients. Natural killer-like (NKL) T cells express on their surface both the T-cell antigen receptor (TCR) and a diverse set of NK-cell receptors (NKR) and share properties of both T cells and NK cells. ζ-Chain phosphorylation is an early event that follows TCR activation or some NKR activation. The ζ-chain of both T cell and NK cells is downregulated in many chronic inflammatory states, HD included. In the present study, NKL T-cell percentage and ζ-chain expression in HD patients were evaluated. Methods: Thirty-three stable HD patients and 30 healthy volunteers were enrolled into the study. NKL T-cell percentage and NKL T-cell ζ-chain mean fluorescence intensity (MFI) were evaluated with flow cytometry. The inflammatory markers C-reactive protein, interleukin-6 and tumour necrosis factor-α were measured in the serum by means of enzyme-linked immunosorbent assay. Results: All the evaluated markers of inflammation were increased in HD patients. In these patients, NKL T-cell percentage (1.71 ± 1.69% vs 3.94 ± 3.86%) and ζ-chain MFI (3.66 ± 2.79 vs 7.03 ± 7.91) were decreased. Conclusions: NKL T-cell percentage and ζ-chain expression is decreased in HD patients. Taking into consideration the continuously increasing age of the HD patients and that normally NKL T-cell numbers increase with age counteracting the impaired T-cell and NK-cell function accompanying advancing age, the above NKL T-cell disturbances could contribute to the impaired immune response in this population. Measures towards alleviating chronic inflammation could partially restore NKL T-cell impairment.