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Discovery based high resolution MS/MS analysis for selection of allergen markers in chocolate and broth powder matrices
- Source :
- Food Chemistry, Food Chemistry, Elsevier, 2021, 343, pp.128533. ⟨10.1016/j.foodchem.2020.128533⟩, Food Chemistry, 2021, 343, pp.128533. ⟨10.1016/j.foodchem.2020.128533⟩, Food chemistry (2021). doi:10.1016/j.foodchem.2020.128533, info:cnr-pdr/source/autori:Pilolli, Rosa; Van Poucke, Christof; De Angelis, Elisabetta; Nitride, Chiara; de Loose, Marc; Gillard, Nathalie; Huet, Anne Catherine; Tranquet, Olivier; Larré, Colette; Adel-Patient, Karine; Bernard, Hervé; Mills, E. N.Clare; Monaci, Linda/titolo:Discovery based high resolution MS%2FMS analysis for selection of allergen markers in chocolate and broth powder matrices/doi:10.1016%2Fj.foodchem.2020.128533/rivista:Food chemistry/anno:2021/pagina_da:/pagina_a:/intervallo_pagine:/volume
- Publication Year :
- 2021
- Publisher :
- HAL CCSD, 2021.
-
Abstract
- International audience; Peptide marker identification is an important step in development of a mass spectrometry method for multiple allergen detection, since specificity, robustness and sensitivity of the overall analytical method will depend on the reliability of the proteotypic peptides. As part of the development of a multi-analyte reference method, discovery analysis of two incurred food matrices has been undertaken to select the most reliable peptide markers. Six allergenic ingredients (milk, egg, peanut, soybean, hazelnut, and almond) were incurred into either chocolate or broth powder matrix. Different conditions of protein extraction and purification were tested and the tryptic peptide pools were analysed by untargeted high resolution tandem mass spectrometry and the resulting fragmentation spectra were processed via a commercial software for sequence identification. The analysis performed on incurred foods provides both a prototype effective and straightforward sample preparation protocol and delivers reliable peptides to be included in a standardized selected reaction monitoring method.
- Subjects :
- ThRAll
[SDV]Life Sciences [q-bio]
High resolution
Peptide
Mass spectrometry
Tandem mass spectrometry
medicine.disease_cause
Discovery analysis
01 natural sciences
Analytical Chemistry
0404 agricultural biotechnology
Allergen
Tandem Mass Spectrometry
Protein purification
medicine
Animals
Sample preparation
Chocolate
Peptide markers
chemistry.chemical_classification
Chromatography
Incurred matrix
010401 analytical chemistry
Selected reaction monitoring
Reproducibility of Results
04 agricultural and veterinary sciences
General Medicine
Allergens
040401 food science
0104 chemical sciences
chemistry
Food allergens
Powders
Food Analysis
Food Science
Subjects
Details
- Language :
- English
- ISSN :
- 03088146 and 18737072
- Database :
- OpenAIRE
- Journal :
- Food Chemistry, Food Chemistry, Elsevier, 2021, 343, pp.128533. ⟨10.1016/j.foodchem.2020.128533⟩, Food Chemistry, 2021, 343, pp.128533. ⟨10.1016/j.foodchem.2020.128533⟩, Food chemistry (2021). doi:10.1016/j.foodchem.2020.128533, info:cnr-pdr/source/autori:Pilolli, Rosa; Van Poucke, Christof; De Angelis, Elisabetta; Nitride, Chiara; de Loose, Marc; Gillard, Nathalie; Huet, Anne Catherine; Tranquet, Olivier; Larré, Colette; Adel-Patient, Karine; Bernard, Hervé; Mills, E. N.Clare; Monaci, Linda/titolo:Discovery based high resolution MS%2FMS analysis for selection of allergen markers in chocolate and broth powder matrices/doi:10.1016%2Fj.foodchem.2020.128533/rivista:Food chemistry/anno:2021/pagina_da:/pagina_a:/intervallo_pagine:/volume
- Accession number :
- edsair.doi.dedup.....ed1cc60a7164a3a4fc3e97bfd5ab1a30