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Biofilm disruption enhances growth rate and carbohydrate-active enzyme production in anaerobic fungi
- Source :
- Bioresource Technology. 358:127361
- Publication Year :
- 2022
- Publisher :
- Elsevier BV, 2022.
-
Abstract
- Anaerobic gut fungi (AGF) are lignocellulose degraders that naturally form biofilms in the rumen of large herbivores and in standard culture techniques. While biofilm formation enhances biomass degradation and carbohydrate-active enzyme (CAZyme) production in some bacteria and aerobic fungi, gene expression and metabolism in AGF biofilms have not been compared to non-biofilm cultures. Here, using the tunable morphology of the non-rhizoidal AGF, Caecomyces churrovis, the impacts of biofilm formation on AGF gene expression, metabolic flux, growth rate, and xylan degradation rate are quantified to inform future industrial scale-up efforts. Contrary to previous findings, C. churrovis upregulated catabolic CAZymes in stirred culture relative to biofilm culture. Using a de novo transcriptome, 197 new transcripts with predicted CAZyme function were identified. Stirred cultures grew and degraded xylan significantly faster than biofilm-forming cultures with negligible differences in primary metabolic flux, offering a way to accelerate AGF biomass valorization without altering the fermentation product profile. The rhizoidal AGF, Neocallimastix lanati, also grew faster with stirring on a solid plant substrate, suggesting that the advantages of stirred C. churrovis cultures may apply broadly to other AGF.
Details
- ISSN :
- 09608524
- Volume :
- 358
- Database :
- OpenAIRE
- Journal :
- Bioresource Technology
- Accession number :
- edsair.doi.dedup.....ed21e7fcf383c57f056da37861e7c14c
- Full Text :
- https://doi.org/10.1016/j.biortech.2022.127361