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Anti-diabetic effect of oligosaccharides from seaweed Sargassum confusum via JNK-IRS1/PI3K signalling pathways and regulation of gut microbiota

Authors :
Shanshan Lai
Xinhua Chen
Chengfeng Yang
Chao Ai
Yihan Chen
Chao Zhao
Xuzhi Wan
Dan Liu
Luying Gao
Bin Liu
Source :
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 131
Publication Year :
2019

Abstract

Brown seaweed Sargassum confusum (C. Agardh) has been used in traditional Chinese medicine to treat a variety of diseases. The aim of the present study was to evaluate the anti-diabetic effect of oligosaccharides from brown seaweed S. confusum (SCO). The anti-diabetic effect of SCO was evaluated in vivo using high-fat/high-sucrose fed hamsters. Molecular mechanisms of modulating gene expression of specific members of insulin signaling pathways were determined. The components of the intestinal microflora in diabetic animals were also analyzed by high-throughput 16S rRNA gene sequencing. And it was found that SCO had a sequence of sulfated anhydrogalactose and methyl sulfated galactoside units. Fasting blood glucose levels were significantly decreased after SCO administration. Histology showed that SCO could protect the cellular architecture of the liver. SCO could also significantly increase the relative abundance of Lactobacillus and Clostridium XIVa and decrease that of Allobaculum, Bacteroides and Clostridium IV. The active role of SCO in anti-diabetic effect was revealed by its regulation of insulin receptor substrate 1/phosphatidylinositol 3-kinase and c-Jun N-terminal kinase pathways. These results suggested that SCO might be used as a functional material to regulate gut microbiota in obese and diabetic individuals.

Details

ISSN :
18736351
Volume :
131
Database :
OpenAIRE
Journal :
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
Accession number :
edsair.doi.dedup.....ed873a7f4d1a86b83bbff950e8add146