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In vivo, competitive blockade of N-methyl-D-aspartate receptors induces rapid changes in filamentous actin and drebrin A distributions within dendritic spines of adult rat cortex
- Source :
- Neuroscience. 140(4)
- Publication Year :
- 2006
-
Abstract
- In vitro studies have demonstrated that prolonged N-methyl-d-aspartate receptor (NMDAR) blockade triggers a homeostatic up-regulation of NMDARs at synapses. Such upregulation can also be seen within 30 min in vivo in adult rats, implicating trafficking of reserve pools of NMDARs. Here, we evaluated the involvement of filamentous actin (F-actin), the major cytoskeletal component in spines, in this rapid in vivo homeostatic response, using biotinylated phalloidin as its probe. We also immuno-labeled spines for drebrin A, an F-actin-binding protein found at excitatory synapses and with a proposed role of modulating F-actin’s cross-linking with one another and interactions with NMDARs. Quantitative 2-D analysis of ultrastructural images revealed that NMDAR blockade increased filamentous actin labeling per spine by 62.5% (P
- Subjects :
- Male
Dendritic spine
Phalloidin
Dendritic Spines
Biology
Filamentous actin
Binding, Competitive
Receptors, N-Methyl-D-Aspartate
Article
Rats, Sprague-Dawley
chemistry.chemical_compound
Animals
Cytoskeleton
Cerebral Cortex
General Neuroscience
Neuropeptides
Age Factors
Long-term potentiation
Dendritic filopodia
Cell biology
Rats
Actin Cytoskeleton
chemistry
nervous system
Excitatory postsynaptic potential
Neuroscience
Postsynaptic density
Excitatory Amino Acid Antagonists
Subjects
Details
- ISSN :
- 03064522
- Volume :
- 140
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Neuroscience
- Accession number :
- edsair.doi.dedup.....ed8ef4d801d6597de73ca0254e25b9c8