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Developmentally regulated H2Av buffering via dynamic sequestration to lipid droplets in Drosophila embryos
- Source :
- eLife, eLife, Vol 7 (2018)
- Publication Year :
- 2018
- Publisher :
- eLife Sciences Publications, Ltd, 2018.
-
Abstract
- Regulating nuclear histone balance is essential for survival, yet in early Drosophila melanogaster embryos many regulatory strategies employed in somatic cells are unavailable. Previous work had suggested that lipid droplets (LDs) buffer nuclear accumulation of the histone variant H2Av. Here, we elucidate the buffering mechanism and demonstrate that it is developmentally controlled. Using live imaging, we find that H2Av continuously exchanges between LDs. Our data suggest that the major driving force for H2Av accumulation in nuclei is H2Av abundance in the cytoplasm and that LD binding slows nuclear import kinetically, by limiting this cytoplasmic pool. Nuclear H2Av accumulation is indeed inversely regulated by overall buffering capacity. Histone exchange between LDs abruptly ceases during the midblastula transition, presumably to allow canonical regulatory mechanisms to take over. These findings provide a mechanistic basis for the emerging role of LDs as regulators of protein homeostasis and demonstrate that LDs can control developmental progression.
- Subjects :
- 0301 basic medicine
Embryo, Nonmammalian
lipid droplets
Gene Dosage
Cytoplasmic Streaming
Midblastula
Histones
Lipid droplet
Drosophila Proteins
Biology (General)
biology
D. melanogaster
Chemistry
General Neuroscience
Gene Expression Regulation, Developmental
General Medicine
Cell biology
Histone
Drosophila melanogaster
Medicine
H2Av
Research Article
QH301-705.5
Science
Active Transport, Cell Nucleus
Embryonic Development
Histone exchange
Models, Biological
General Biochemistry, Genetics and Molecular Biology
Chromosomes
03 medical and health sciences
Drosophila embryos
Animals
Blastoderm
Interphase
protein buffering
Cell Nucleus
General Immunology and Microbiology
Cell Biology
biology.organism_classification
Kinetics
030104 developmental biology
Cytoplasm
biology.protein
Nuclear transport
Developmental biology
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 2050084X
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- eLife
- Accession number :
- edsair.doi.dedup.....edabbd3267e76bf0a894727c0658b7fe