Back to Search
Start Over
Uptake and intracellular distribution of different types of nanoparticles in primary human myoblasts and myotubes
- Publication Year :
- 2019
-
Abstract
- The use of nanoparticles as drug carriers in the field of skeletal muscle diseases has been poorly addressed and the interaction of nanoparticles with skeletal muscle cells has been investigated almost exclusively on C2C12 murine myoblasts. In this study we investigated the effects poly(lactide-co-glycolide) nanoparticles, mesoporous silica nanoparticles and liposomes, on the viability of primary human myoblasts and analyzed their cellular uptake and intracellular distribution in both primary human myoblasts and myotubes. Our data demonstrate that poly(lactide-co-glycolide) nanoparticles do not negatively affect myoblasts viability, contrarily to mesoporous silica nanoparticles and liposomes that induce a decrease in cell viability at the highest doses and longest incubation time. Poly(lactide-co-glycolide) nanoparticles and mesoporous silica nanoparticles are internalized by endocytosis, poly(lactide-co-glycolide) nanoparticles undergo endosomal escape whereas mesoporous silica nanoparticles always occur within vacuoles. Liposomes were rarely observed within the cells. The uptake of all tested nanoparticles was less prominent in primary human myotubes as compared to myoblasts. Our findings represent the first step toward the characterization of the interaction between nanoparticles and primary human muscle cells and suggest that poly(lactide-co-glycolide) nanoparticles might find an application for drug delivery to skeletal muscle.
- Subjects :
- Time Factors
Cell Survival
Muscle Fibers, Skeletal
Pharmaceutical Science
02 engineering and technology
030226 pharmacology & pharmacy
Myoblasts
03 medical and health sciences
0302 clinical medicine
Drug Delivery Systems
Nanoparticle
medicine
Electron microscopy
Myocyte
Humans
Polyglactin 910
Cells, Cultured
Fluorescence microscopy
Liposome
Dose-Response Relationship, Drug
Myogenesis
Chemistry
Primary human myoblast
Skeletal muscle
Primary human myotube
Mesoporous silica
021001 nanoscience & nanotechnology
Silicon Dioxide
Endocytosis
medicine.anatomical_structure
Drug delivery
Liposomes
Biophysics
Nanoparticles
0210 nano-technology
Drug carrier
C2C12
Porosity
3003
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....ee188f3685ae033ad914547c56be690d