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Biosynthesis of Silver Nanoparticles from Oropharyngeal Candida glabrata Isolates and Their Antimicrobial Activity against Clinical Strains of Bacteria and Fungi
- Source :
- Nanomaterials, Volume 8, Issue 8, Nanomaterials, Vol 8, Iss 8, p 586 (2018)
- Publication Year :
- 2018
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2018.
-
Abstract
- The objective of the present study was one step extracellular biosynthesis of silver nanoparticles (AgNPs) using supernatant of Candida glabrata isolated from oropharyngeal mucosa of human immunodeficiency virus (HIV) patients and evaluation of their antibacterial and antifungal potential against human pathogenic bacteria and fungi. The mycosynthesized AgNPs were characterized by color visualization, ultraviolet-visible (UV) spectroscopy, fourier transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). The FTIR spectra revealed the binding and stabilization of nanoparticles with protein. The TEM analysis showed that nanoparticles were well dispersed and predominantly spherical in shape within the size range of 2&ndash<br />15 nm. The antibacterial and antifungal potential of AgNPs were characterized by determining minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC)/ minimum fungicidal concentration (MFC), and well diffusion methods. The MBC and MFC were found in the range of 62.5&ndash<br />250 &mu<br />g/mL and 125&ndash<br />500 &mu<br />g/mL, which revealed that bacterial strains were more susceptible to AgNPs than fungal strains. These differences in bactericidal and fungicidal concentrations of the AgNPs were due to the differences in the cell structure and organization of bacteria and yeast cells. The interaction of AgNPs with C. albicans analyzed by TEM showed the penetration of nanoparticles inside the Candida cells, which led the formation of &ldquo<br />pits&rdquo<br />and &ldquo<br />pores&rdquo<br />that result from the rupturing of the cell wall and membrane. Further, TEM analysis showed that Candida cells treated with AgNPs were highly deformed and the cells had shrunken to a greater extent because of their interaction with the fungal cell wall and membrane, which disrupted the structure of the cell membrane and inhibited the normal budding process due to the destruction and loss of membrane integrity and formation of pores that may led to the cell death.
- Subjects :
- 0301 basic medicine
MBC
General Chemical Engineering
MFC
extracellular
Candida glabrata
02 engineering and technology
Silver nanoparticle
Microbiology
lcsh:Chemistry
Cell wall
Cell membrane
03 medical and health sciences
Minimum inhibitory concentration
medicine
General Materials Science
MIC
Minimum bactericidal concentration
biology
Chemistry
021001 nanoscience & nanotechnology
biology.organism_classification
Antimicrobial
mycosynthesis
030104 developmental biology
medicine.anatomical_structure
lcsh:QD1-999
FTIR
membrane integrity
TEM
0210 nano-technology
Bacteria
Subjects
Details
- Language :
- English
- ISSN :
- 20794991
- Database :
- OpenAIRE
- Journal :
- Nanomaterials
- Accession number :
- edsair.doi.dedup.....ee68082e6e9fdedd1f5bccbdf68e1bf5
- Full Text :
- https://doi.org/10.3390/nano8080586