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Conserved miRNAs modulate the expression of potential transcription factors of isoflavonoid biosynthetic pathway in soybean seeds
- Source :
- Molecular Biology Reports. 46:3713-3730
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Despite the significant importance of soybean isoflavone, the regulatory mechanism of miRNAs during its biosynthesis is highly unexplored. In the present work, nine existing miRNAs along with their ten corresponding target genes were identified and validated in soybean for their possible role during isoflavonoid biosynthesis and accumulation. Temporal expression analysis at four key stages of seed development (35, 45, 55 and 65DAF) of all the miRNA-target pairs showed varying degree of differential accumulation in two soybean genotypes (NRC37: high isoflavone; and NRC7: low isoflavone). Differential expression of MYB65-Gma-miR159, MYB96-Gma-miRNA1534, MYB176-Gma-miRNA5030, SPL9-Gma-miRNA156, TCP3, TCP4-Gma-miRNA319, WD40-Gma-miRNA162, UDP-glucose: flavonoid 3-O-glucosyltransferase-Gma-miRNA396, and CHI3-Gma-miRNA5434 showed an important relationship with their targets in both the soybean genotypes across all the stages. Therefore, the finding of the present work would certainly increase our understanding of molecular regulation of isoflavone biosynthetic pathway mediated by the miRNA which would guide molecular breeder to develop isoflavone rich soybean cultivars.
- Subjects :
- 0301 basic medicine
Genotype
Flavonoid
Biology
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Isoflavonoid
Biosynthesis
Gene Expression Regulation, Plant
microRNA
Genetics
MYB
Molecular Biology
Gene
Transcription factor
chemistry.chemical_classification
food and beverages
General Medicine
Isoflavones
Biosynthetic Pathways
MicroRNAs
030104 developmental biology
Biochemistry
chemistry
030220 oncology & carcinogenesis
Isoflavonoid biosynthesis
Seeds
Soybeans
Transcription Factors
Subjects
Details
- ISSN :
- 15734978 and 03014851
- Volume :
- 46
- Database :
- OpenAIRE
- Journal :
- Molecular Biology Reports
- Accession number :
- edsair.doi.dedup.....ee7734f0fa93c77a49842fa485e8e6af