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Establishment of a nasopharyngeal carcinoma cell line capable of undergoing lytic Epstein-Barr virus reactivation
- Source :
- Laboratory investigation; a journal of technical methods and pathology. 98(8)
- Publication Year :
- 2017
-
Abstract
- Epstein-Barr virus (EBV) infects more than 90% of the adult human population. Undifferentiated nasopharyngeal carcinoma (NPC) is common in Southeast Asia, with a particularly high incidence among southern Chinese. The EBV genome can be detected in practically all cancer cells in undifferentiated NPC. The role of EBV in pathogenesis of undifferentiated NPC remains elusive. NPC cell lines are known to be difficult to establish in culture. The EBV+ve NPC cell lines, even if established in culture, rapidly lost their EBV episomes upon prolonged propagation. At present, the C666-1 NPC cell line, which is defective in lytic EBV reactivation, is the only EBV+ve NPC cell line available for NPC and EBV research. The need to establish new and representative NPC cell lines is eminent for NPC and EBV research. In this study, we report the use of the Rho-associated kinase inhibitor (Y-27632) has facilitated the establishment of a new EBV+ve NPC cell line from an earlier established NPC xenograft, C17. The C17 cell line was tumorigenic in immune-deficient mice (NOD/SCID). It retained the EBV episomes and could be induced to undergo productive lytic reactivation of EBV to generate infectious virus particles. The C17 cell line represents a new investigative tool for NPC and EBV studies. The ability of C17 to undergo lytic reactivation is unique and opens up the opportunity to examine regulation of latent and lytic infection of EBV and their contributions to NPC pathogenesis.
- Subjects :
- 0301 basic medicine
Epstein-Barr Virus Infections
Herpesvirus 4, Human
Population
Transplantation, Heterologous
Mice, Nude
Genome, Viral
Mice, SCID
Biology
medicine.disease_cause
Virus
Pathology and Forensic Medicine
03 medical and health sciences
Mice, Inbred NOD
hemic and lymphatic diseases
Cell Line, Tumor
otorhinolaryngologic diseases
medicine
Animals
Humans
education
Molecular Biology
education.field_of_study
Mice, Inbred BALB C
Nasopharyngeal Carcinoma
Nasopharyngeal Neoplasms
Cell Biology
medicine.disease
Epstein–Barr virus
Tumor Burden
Transplantation
stomatognathic diseases
030104 developmental biology
Nasopharyngeal carcinoma
Lytic cycle
Cell culture
Karyotyping
Cancer cell
Host-Pathogen Interactions
Cancer research
Virus Activation
Subjects
Details
- ISSN :
- 15300307
- Volume :
- 98
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Laboratory investigation; a journal of technical methods and pathology
- Accession number :
- edsair.doi.dedup.....eea1c636ea8d2e68403580c2fcd57b75