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Effect of a polysaccharide from Poria cocos on humoral response in mice immunized by H1N1 influenza and HBsAg vaccines
- Source :
- International Journal of Biological Macromolecules. 91:248-257
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Poria cocos has a long history of medicinal use in China. Polysaccharides and their derivatives in the medicine exhibit many beneficial biological activities including anticancer, anti-inflammatory, antioxidant and antiviral activities. In this study, a new polysaccharide (PCP-II) was isolated from sclerotium of Poria cocos. Its physico-chemical characters were identified and its adjuvant activity was investigated in mice co-immunized with H1N1 influenza vaccine and hepatitis B surface antigen (HBsAg). The results revealed that PCP-II has a molecular weight of 29.0kDa. It was composed of fucose, mannose, glucose and galactose in molar ration of 1.00:1.63:0.16:6.29 respectively. Pharmacological data demonstrated that PCP-II increased antigen-specific antibody levels in mice immunized with influenza vaccine. PCP-II also elicited anti-HBsAg antibodies at significantly higher titers and generated robust and durable immunity compared to mice immunized with HBsAg-alum following two administrations. PCP-II improved proliferation of splenocytes, stimulated IL-12p70 and TNF-α productions in dendritic cells and macrophages respectively. These results suggested that PCP-II-adjuvanted vaccines enhanced humoral and cellular immunity. PCP-II could be developed as an efficacious adjuvant in human and animal vaccines.
- Subjects :
- 0301 basic medicine
Cellular immunity
HBsAg
Influenza vaccine
medicine.medical_treatment
Biochemistry
Fucose
Microbiology
Mice
03 medical and health sciences
chemistry.chemical_compound
Influenza A Virus, H1N1 Subtype
0302 clinical medicine
Structural Biology
Immunity
medicine
Animals
Humans
Hepatitis B Vaccines
Molecular Biology
Hepatitis B Surface Antigens
biology
Tumor Necrosis Factor-alpha
Fungal Polysaccharides
General Medicine
Interleukin-12
Virology
Immunity, Humoral
030104 developmental biology
chemistry
Influenza Vaccines
030220 oncology & carcinogenesis
biology.protein
Interleukin 12
Immunization
Antibody
Adjuvant
Wolfiporia
Subjects
Details
- ISSN :
- 01418130
- Volume :
- 91
- Database :
- OpenAIRE
- Journal :
- International Journal of Biological Macromolecules
- Accession number :
- edsair.doi.dedup.....eeaa63ae28418896743340ef9d71a74b