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Pilose antler peptide protects osteoblasts from inflammatory and oxidative injury through EGF/EGFR signaling
- Source :
- International Journal of Biological Macromolecules. 99:15-20
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Epidermal growth factor (EGF)/EFG receptor (EGFR) signaling plays an important role in the osteoblastogenesis. The potential effects of pilose antler peptide (PAP) on osteoblast cell damages was investigated in our present study through EGF/EGFR signaling. In MC3T3-E1 osteoblastic cells, PAP treatment significantly inhibited the production of inflammatory cytokines by decreasing the levels of serum proinflammatory cytokines interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). PAP treatment also alleviated the oxidative responses as indicated by increased activities of catalase (SOD) and decreased levels of malondialdehyde (MDA). EGF inhibition, by siRNA knockdown, almost abolished PAP-induced osteoblast cytoprotection against inflammation and oxidant stress. Further, our results showed that PAP stimulated the nuclear erythroid factor 2-related factor 2 (Nrf2)2/heme oxygenase-1(HO-1) signaling, and inhibited the activation of uclear factor kappa B (NF-κB) pathway in MC3T3-E1 cells. On the other hand, EGF siRNA knockdown inhibited PAP-induced cytoprotection, which decreased the expression of Nrf-2, HO-1 and increased the level of p-NF-κBp65, p-IκBα in MC3T3-E1 cells. Thus, our research demonstrated that PAP protects osteoblasts from inflammatory and oxidative injury through EGF/EGFR signaling.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Cell Survival
Antlers
Inflammation
medicine.disease_cause
Biochemistry
Cell Line
Proinflammatory cytokine
Mice
03 medical and health sciences
0302 clinical medicine
Structural Biology
Epidermal growth factor
Malondialdehyde
Internal medicine
medicine
Animals
Molecular Biology
Osteoblasts
Epidermal Growth Factor
Superoxide Dismutase
Chemistry
Deer
Osteoblast
General Medicine
Cytoprotection
ErbB Receptors
Oxidative Stress
030104 developmental biology
medicine.anatomical_structure
Endocrinology
030220 oncology & carcinogenesis
Cytokines
Tumor necrosis factor alpha
medicine.symptom
Signal transduction
Peptides
Oxidative stress
Signal Transduction
Subjects
Details
- ISSN :
- 01418130
- Volume :
- 99
- Database :
- OpenAIRE
- Journal :
- International Journal of Biological Macromolecules
- Accession number :
- edsair.doi.dedup.....eec0a0171e83f8ef0a6bf5d7120e1327