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Efficacy of Lytic Phage Cocktails on Staphylococcus aureus and Pseudomonas aeruginosa in Mixed-Species Planktonic Cultures and Biofilms

Authors :
Legesse Garedew Kifelew
Peter Speck
David L. Gordon
Sandra Morales
James G. Mitchell
Morgyn S. Warner
Nicky Thomas
Kifelew, Legesse Garedew
Warner, Morgyn S
Morales, Sandra
Thomas, Nicky
Gordon, David L
Mitchell, James G
Speck, Peter G
Source :
Viruses, Volume 12, Issue 5, Viruses, Vol 12, Iss 559, p 559 (2020)
Publication Year :
2020
Publisher :
Multidisciplinary Digital Publishing Institute, 2020.

Abstract

The efficacy of phages in multispecies infections has been poorly examined. The in vitro lytic efficacies of phage cocktails AB-SA01, AB-PA01, which target Staphylococcus aureus and Pseudomonas aeruginosa, respectively, and their combination against their hosts were evaluated in S. aureus and P. aeruginosa mixed-species planktonic and biofilm cultures. Green fluorescent protein (GFP)-labelled P. aeruginosa PAO1 and mCherry-labelled S. aureus KUB7 laboratory strains and clinical isolates were used as target bacteria. During real-time monitoring using fluorescence spectrophotometry, the density of mCherry S. aureus KUB7 and GFP P. aeruginosa PAO1 significantly decreased when treated by their respective phage cocktail, a mixture of phage cocktails, and gentamicin. The decrease in bacterial density measured by relative fluorescence strongly associated with the decline in bacterial cell counts. This microplate-based mixed-species culture treatment monitoring through spectrophotometry combine reproducibility, rapidity, and ease of management. It is amenable to high-throughput screening for phage cocktail efficacy evaluation. Each phage cocktail, the combination of the two phage cocktails, and tetracycline produced significant biofilm biomass reduction in mixed-species biofilms. This study result shows that these phage cocktails lyse their hosts in the presence of non-susceptible bacteria. These data support the use of phage cocktails therapy in infections with multiple bacterial species.

Details

Language :
English
ISSN :
19994915
Database :
OpenAIRE
Journal :
Viruses
Accession number :
edsair.doi.dedup.....eed92b90e06dc15d2d5984a2351ba1b3
Full Text :
https://doi.org/10.3390/v12050559