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Efficacy of Lytic Phage Cocktails on Staphylococcus aureus and Pseudomonas aeruginosa in Mixed-Species Planktonic Cultures and Biofilms
- Source :
- Viruses, Volume 12, Issue 5, Viruses, Vol 12, Iss 559, p 559 (2020)
- Publication Year :
- 2020
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2020.
-
Abstract
- The efficacy of phages in multispecies infections has been poorly examined. The in vitro lytic efficacies of phage cocktails AB-SA01, AB-PA01, which target Staphylococcus aureus and Pseudomonas aeruginosa, respectively, and their combination against their hosts were evaluated in S. aureus and P. aeruginosa mixed-species planktonic and biofilm cultures. Green fluorescent protein (GFP)-labelled P. aeruginosa PAO1 and mCherry-labelled S. aureus KUB7 laboratory strains and clinical isolates were used as target bacteria. During real-time monitoring using fluorescence spectrophotometry, the density of mCherry S. aureus KUB7 and GFP P. aeruginosa PAO1 significantly decreased when treated by their respective phage cocktail, a mixture of phage cocktails, and gentamicin. The decrease in bacterial density measured by relative fluorescence strongly associated with the decline in bacterial cell counts. This microplate-based mixed-species culture treatment monitoring through spectrophotometry combine reproducibility, rapidity, and ease of management. It is amenable to high-throughput screening for phage cocktail efficacy evaluation. Each phage cocktail, the combination of the two phage cocktails, and tetracycline produced significant biofilm biomass reduction in mixed-species biofilms. This study result shows that these phage cocktails lyse their hosts in the presence of non-susceptible bacteria. These data support the use of phage cocktails therapy in infections with multiple bacterial species.
- Subjects :
- 0301 basic medicine
Staphylococcus aureus
Tetracycline
030106 microbiology
efficacy
lcsh:QR1-502
Colony Count, Microbial
medicine.disease_cause
Bacterial cell structure
lcsh:Microbiology
Article
biofilm
Microbiology
03 medical and health sciences
planktonic culture
Virology
Drug Resistance, Bacterial
medicine
Bacteriophages
mixed-species culture
biology
Pseudomonas aeruginosa
Chemistry
fungi
Biofilm
Reproducibility of Results
biology.organism_classification
Coculture Techniques
Anti-Bacterial Agents
030104 developmental biology
Infectious Diseases
Lytic cycle
Biofilms
Gentamicin
fluorescence
phage cocktail therapy
Bacteria
mixed-speciesculture
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 19994915
- Database :
- OpenAIRE
- Journal :
- Viruses
- Accession number :
- edsair.doi.dedup.....eed92b90e06dc15d2d5984a2351ba1b3
- Full Text :
- https://doi.org/10.3390/v12050559