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Efficient intracellular retrotransposition of an exogenous primate retrovirus genome
- Source :
- Scopus-Elsevier
- Publication Year :
- 2000
- Publisher :
- Wiley, 2000.
-
Abstract
- The foamy virus (FV) subgroup of Retroviridae reverse transcribe their RNA (pre-)genome late in the replication cycle before leaving an infected cell. We studied whether a marker gene-transducing FV vector is able to shuttle to the nucleus and integrate into host cell genomic DNA. While a potential intracellular retrotransposition of vectors derived from other retroviruses was below the detection limit of our assay, we found that up to 5% of cells transfected with the FV vector were stably transduced, harboring 1 to approximately 10 vector integrants. Generation of the integrants depended on expression of functional capsid, reverse transcriptase and integrase proteins, and did not involve an extracellular step. PCR analysis of the U3 region of the 5' long terminal repeat and determination of proviral integration sites showed that a reverse transcription step had taken place to generate the integrants. Co-expression of a mutated envelope allowing particle egress and avoiding extracellular infection resulted in a significantly increased rescue of cells harboring integrants, suggesting that accumulation of proviruses via intracellular retrotransposition represents an integral part of the FV replication strategy.
- Subjects :
- Primates
Retroelements
Genetic Vectors
Retrotransposon
Genome, Viral
General Biochemistry, Genetics and Molecular Biology
Animals
Humans
Vector (molecular biology)
Primate Retrovirus
Spumavirus
Molecular Biology
DNA Primers
Base Sequence
General Immunology and Microbiology
biology
General Neuroscience
Gene Products, env
RNA
Articles
biology.organism_classification
Virology
Reverse transcriptase
Long terminal repeat
Integrase
biology.protein
RNA, Viral
HeLa Cells
Subjects
Details
- ISSN :
- 14602075
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- The EMBO Journal
- Accession number :
- edsair.doi.dedup.....eeec2d2d0bfec2c8faeaa3d7d793eb80