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Molecular pathology analyses of two fatal human infections of avian influenza A(H7N9) virus

Authors :
Ye Zheng
Yanling Feng
Anli Zhang
Qingguo Chen
Yunwen Hu
Dong Zeng
Zhiyong Zhang
Lvyin Hu
Jun Zhang
Liang Chen
Shuihua Lu
Source :
Journal of Clinical Pathology. 68:57-63
Publication Year :
2014
Publisher :
BMJ, 2014.

Abstract

Aims To investigate the histopathological manifestations of two fatal cases of H7N9 influenza A virus infection. Methods Pulmonary and hepatic specimens from two fatal cases of H7N9 influenza virus infection were examined using H&E staining. Additionally, in situ hybridisation was performed with probes (ViewRNA) targeting H7N9 RNA and IP-10, interleukin (IL)-6 mRNA. The distribution of surfactant protein A (SP-A), surfactant protein B (SP-B), CD3, CD4, CD8, CD68 and C4d were determined with immunohistochemistry. Results Apart from the typical diffuse alveolar damage and hyaline membrane observed in severe influenza infection, we detected H7N9 RNA and massive intrapulmonary production of IP-10 and IL-6 mRNA using in situ hybridisation. Hyperplasia of type II pneumocytes was observed by H&E staining and immunohistochemistry. Proliferating macrophages and clustered neutrophils in the infected lungs were observed, whereas T lymphocytes, especially CD4T helper cells, were markedly depleted. No obvious complement deposition was found in lung tissues. Conclusions Our findings suggest that H7N9 influenza virus induced an immunological response towards overt pulmonary inflammation and systemic lymphopenia which led to intense alveolar damage and respiratory failure.

Details

ISSN :
14724146 and 00219746
Volume :
68
Database :
OpenAIRE
Journal :
Journal of Clinical Pathology
Accession number :
edsair.doi.dedup.....eef11b2dbbf4faee9abd7a1568913166