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ASIC1a contributes to the symptom of pain in a rat model of chronic prostatitis
- Source :
- Asian Journal of Andrology, Asian Journal of Andrology, Vol 20, Iss 3, Pp 300-305 (2018)
- Publication Year :
- 2018
- Publisher :
- Medknow, 2018.
-
Abstract
- This study aims to validate our hypothesis that acid-sensing ion channels (ASICs) may contribute to the symptom of pain in patients with chronic prostatitis (CP). We first established a CP rat model, then isolated the L5-S2 spinal dorsal horn neurons for further studies. ASIC1a was knocked down and its effects on the expression of neurogenic inflammation-related factors in the dorsal horn neurons of rat spinal cord were evaluated. The effect of ASIC1a on the Ca2+ ion concentration in the dorsal horn neurons of rat spinal cord was measured by the intracellular calcium ([Ca2+]i) intensity. The effect of ASIC1a on the p38/mitogen-activated protein kinase (MAPK) signaling pathway was also determined. ASIC1a was significantly upregulated in the CP rat model as compared with control rats. Acid-induced ASIC1a expression increased [Ca2+]i intensity in the dorsal horn neurons of rat spinal cord. ASIC1a also increased the levels of neurogenic inflammation-related factors and p-p38 expression in the acid-treated dorsal horn neurons. Notably, ASIC1a knockdown significantly decreased the expression of pro-inflammatory cytokines. Furthermore, the levels of p-p38 and pro-inflammatory cytokines in acid-treated dorsal horn neurons were significantly decreased in the presence of PcTx-1, BAPTA-AM, or SB203580. Our results showed that ASIC1a may contribute to the symptom of pain in patients with CP, at least partially, by regulating the p38/MAPK signaling pathway.
- Subjects :
- Male
0301 basic medicine
MAPK/ERK pathway
Pyridines
Spider Venoms
lcsh:RC870-923
p38 Mitogen-Activated Protein Kinases
Calcium in biology
0302 clinical medicine
Phosphorylation
Egtazic Acid
Chelating Agents
Gene knockdown
Imidazoles
General Medicine
Prostatitis
Up-Regulation
Posterior Horn Cells
medicine.anatomical_structure
Acid Sensing Ion Channel Blockers
Gene Knockdown Techniques
Cytokines
Original Article
ASIC1a
chronic prostatitis
pain symptom
Signal transduction
medicine.medical_specialty
MAP Kinase Signaling System
Urology
p38 mitogen-activated protein kinases
Pain
03 medical and health sciences
Internal medicine
medicine
Animals
Protein kinase A
Protein Kinase Inhibitors
Inflammation
business.industry
lcsh:Diseases of the genitourinary system. Urology
Spinal cord
medicine.disease
Rats
Acid Sensing Ion Channels
Disease Models, Animal
030104 developmental biology
Endocrinology
nervous system
Chronic Disease
Calcium
Peptides
business
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 1008682X
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Asian Journal of Andrology
- Accession number :
- edsair.doi.dedup.....ef0fa5dccd7a0c2736c9b960855362e1