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A Rapid Assay to Detect Toxigenic Penicillium spp. Contamination in Wine and Musts
- Source :
- Toxins, Toxins, Vol 8, Iss 8, p 235 (2016)
- Publication Year :
- 2016
-
Abstract
- Wine and fermenting musts are grape products widely consumed worldwide. Since the presence of mycotoxin-producing fungi may greatly compromise their quality characteristics and safety, there is an increasing need for relatively rapid “user friendly” quantitative assays to detect fungal contamination both in grapes delivered to wineries and in final products. Although other fungi are most frequently involved in grape deterioration, secondary infections by Penicillium spp. are quite common, especially in cool areas with high humidity and in wines obtained by partially dried grapes. In this work, a single-tube nested real-time PCR approach—successfully applied to hazelnut and peanut allergen detection—was tested for the first time to trace Penicillium spp. in musts and wines. The method consisted of two sets of primers specifically designed to target the β-tubulin gene, to be simultaneously applied with the aim of lowering the detection limit of conventional real-time PCR. The assay was able to detect up to 1 fg of Penicillium DNA. As confirmation, patulin content of representative samples was determined. Most of analyzed wines/musts returned contaminated results at >50 ppb and a 76% accordance with molecular assay was observed. Although further large-scale trials are needed, these results encourage the use of the newly developed method in the pre-screening of fresh and processed grapes for the presence of Penicillium DNA before the evaluation of related toxins.
- Subjects :
- DNA, Bacterial
Health, Toxicology and Mutagenesis
Secondary infection
lcsh:Medicine
Wine
Toxicology
Real-Time Polymerase Chain Reaction
Patulin
chemistry.chemical_compound
0404 agricultural biotechnology
Rapid assay
Vitis
Food science
Mycotoxin
Bacteriological Techniques
biology
Communication
Penicillium detection
lcsh:R
Penicillium
food and beverages
Reproducibility of Results
04 agricultural and veterinary sciences
Contamination
Mycotoxins
biology.organism_classification
grape
040401 food science
food safety
chemistry
Fermentation
Food Microbiology
Subjects
Details
- ISSN :
- 20726651
- Volume :
- 8
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Toxins
- Accession number :
- edsair.doi.dedup.....ef4575e642c88b0dbfa97b5ba2d12ff9