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Multicenter Intestinal Current Measurements in Rectal Biopsies from CF and Non-CF Subjects to Monitor CFTR Function
- Source :
- PLoS ONE, Vol 8, Iss 9, p e73905 (2013), PLoS ONE
- Publication Year :
- 2013
- Publisher :
- Public Library of Science (PLoS), 2013.
-
Abstract
- Intestinal current measurements (ICM) from rectal biopsies are a sensitive means to detect cystic fibrosis transmembrane conductance regulator (CFTR) function, but have not been optimized for multicenter use. We piloted multicenter standard operating procedures (SOPs) to detect CFTR activity by ICM and examined key questions for use in clinical trials. SOPs for ICM using human rectal biopsies were developed across three centers and used to characterize ion transport from non-CF and CF subjects (two severe CFTR mutations). All data were centrally evaluated by a blinded interpreter. SOPs were then used across four centers to examine the effect of cold storage on CFTR currents and compare CFTR currents in biopsies from one subject studied simultaneously either at two sites (24 hours post-biopsy) or when biopsies were obtained by either forceps or suction. Rectal biopsies from 44 non-CF and 17 CF subjects were analyzed. Mean differences (µA/cm(2); 95% confidence intervals) between CF and non-CF were forskolin/IBMX=102.6(128.0 to 81.1), carbachol=96.3(118.7 to 73.9), forskolin/IBMX+carbachol=200.9(243.1 to 158.6), and bumetanide=-44.6 (-33.7 to -55.6) (P
- Subjects :
- Adult
Male
medicine.medical_specialty
IBMX
Carbachol
Cystic Fibrosis
Biopsy
Urology
Cystic Fibrosis Transmembrane Conductance Regulator
lcsh:Medicine
Cold storage
Cystic fibrosis
Young Adult
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Chlorides
Internal medicine
Cyclic AMP
medicine
Humans
lcsh:Science
Aged
030304 developmental biology
0303 health sciences
Multidisciplinary
Forskolin
biology
business.industry
Sodium
lcsh:R
Rectum
Area under the curve
Middle Aged
medicine.disease
Cystic fibrosis transmembrane conductance regulator
Endocrinology
ROC Curve
030228 respiratory system
chemistry
biology.protein
Female
lcsh:Q
business
Bumetanide
Research Article
medicine.drug
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....ef89a9cd98a7b6129904aac7d3cb6ce6