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Specific adsorption of the autoantibodies from rheumatoid arthritis patient plasma using histidine-containing affinity beads
- Source :
- Journal of biomaterials science. Polymer edition. 19(7)
- Publication Year :
- 2008
-
Abstract
- Rheumatoid arthritis is characterized by chronic polyarthritis and destruction of multiple joints. In this study, poly(hydroxyethyl methacrylate-N-methacryloyl-(L-histidine)-methylester) (PHEMAH) beads were used in the removal of pathogenic antibodies from rheumatoid arthritis patient plasma in a packed bed column. PHEMAH beads, in the size range of 80-120 mum, were produced by suspension polymerization. The beads were contacted with blood in an in vitro system. Loss of blood cells and clotting times were followed. PHEMAH beads were characterized by scanning electron microscopy. We found that PHEMAH beads had a spherical shape and porous structure. Loss of cells in the blood contacting with PHEMAH beads was negligible. IgM-antibody adsorption capacity decreased significantly with the increase of the plasma flow-rate. With increasing IgM-antibody concentration, the amount of IgM-antibody adsorbed per unit mass increased and then reached saturation. Maximum IgM-antibody adsorption amount was 69.2 mg/g. IgM-antibody molecules could be repeatedly adsorbed and desorbed without noticeable loss in the IgM-antibody adsorption amount.
- Subjects :
- Materials science
Time Factors
Scanning electron microscope
Biomedical Engineering
Biophysics
Bioengineering
Biomaterials
Arthritis, Rheumatoid
Adsorption
medicine
Humans
Histidine
Immunoadsorption
Polyhydroxyethyl Methacrylate
Autoantibodies
Packed bed
Chromatography
biology
Molecular Structure
medicine.disease
Immunoglobulin M
biology.protein
Microscopy, Electron, Scanning
Polyarthritis
Suspension polymerization
Antibody
Subjects
Details
- ISSN :
- 09205063
- Volume :
- 19
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Journal of biomaterials science. Polymer edition
- Accession number :
- edsair.doi.dedup.....efcf206ea1b039d6576f3b7860ecdd4f