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The protective effect of Gan Shen Fu Fang on liver endothelial cells in common bile duct-ligated rats
- Source :
- World Journal of Traditional Chinese Medicine, Vol 3, Iss 4, Pp 21-25 (2017)
- Publication Year :
- 2017
- Publisher :
- Wolters Kluwer Medknow Publications, 2017.
-
Abstract
- Objective: To elucidate the protective effect of Gan Shen Fu Fang (GSFF) on liver endothelial cells in common bile duct-ligated (CBDL) rats. Materials and Methods: Cirrhosis was induced by common bile duct ligation. The rats were divided into three groups: sham group, CBDL group, and GSFF group. After 2 weeks of ligation, rats in the GSFF group were administered GSFF. After 4 weeks, the hydroxyproline (Hyp) content of liver tissues was spectrophotometrically determined. The histological changes were evaluated by H and E and Masson staining. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were used to observe the ultrastructural changes in the liver, especially in the liver sinusoidal endothelial cells (LSECs). Results: Hyp synthesis was significantly inhibited by GSFF, which agreed with the results from H and E and Masson staining for liver fibrosis. The TEM observations of CBDL rats revealed reduced hepatocyte microvilli and deposited fibrous tissue underneath LSECs. SEM confirmed the TEM findings and showed that the fenestrae of LSECs decreased and even disappeared in CBDL rats. The morphological results indicated hepatic sinusoid capillarization. GSFF promoted the restoration of fenestrae and reversed hepatic sinusoid capillarization. Conclusion: GSFF can inhibit Hyp synthesis, restore the fenestrae of LSECs, and reverse hepatic sinusoid capillarization in CBDL rats. These results provide a basis for future detailed investigations of the mechanism of action of GSFF in LSECs.
- Subjects :
- Pathology
medicine.medical_specialty
Cirrhosis
hepatic sinusoid capillarization
liver sinusoidal endothelial cells
H&E stain
03 medical and health sciences
Hydroxyproline
chemistry.chemical_compound
0302 clinical medicine
medicine
lcsh:R5-920
Common bile duct
Chemistry
medicine.disease
Staining
medicine.anatomical_structure
Complementary and alternative medicine
Mechanism of action
030220 oncology & carcinogenesis
Hepatocyte
030211 gastroenterology & hepatology
medicine.symptom
Gan Shen Fu Fang
Ligation
lcsh:Medicine (General)
Subjects
Details
- Language :
- English
- ISSN :
- 25892894 and 23118571
- Volume :
- 3
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- World Journal of Traditional Chinese Medicine
- Accession number :
- edsair.doi.dedup.....efd9c0466bd89f239cf0029a4b29a08f