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CsBZIP40, a BZIP transcription factor in sweet orange, plays a positive regulatory role in citrus bacterial canker response and tolerance
- Source :
- PLoS ONE, Vol 14, Iss 10, p e0223498 (2019), PLoS ONE
- Publication Year :
- 2019
- Publisher :
- Public Library of Science (PLoS), 2019.
-
Abstract
- Citrus bacterial canker (CBC) caused by Xanthomonas citri subsp. citri (Xcc) is a systemic bacterial disease that affects citrus plantations globally. Biotic stress in plants has been linked to a group of important transcription factors known as Basic Leucine Zippers (BZIPs). In this study, CsBZIP40 was functionally characterized by expression analysis, including induction by Xcc and hormones, subcellular localization, over-expression and RNAi silencing. CsBZIP40 belongs to group D of the CsBZIP family of transcription factors and localizes in the nucleus, potentially serving as a transcriptional regulator. In wild type (WT) plants CsBZIP40 can be induced by plant hormones in addition to infection by Xcc which has given insight into its involvement in CBC. In the present study, over-expression of CsBZIP40 conferred resistance to Xcc while its silencing led to Xcc susceptibility. Both over-expression and RNAi affected salicylic acid (SA) production and expression of the genes involved in the SA synthesis and signaling pathway, in addition to interaction of CsBZIP40 with CsNPR1, as detected by a GST pull-down assay. Taken together, the results of this study confirmed the important role of CsBZIP40 in improving resistance to citrus canker through the SA signaling pathway by the presence of NPR1 to activate PR genes. Our findings are of potential value in the breeding of tolerance to CBC in citrus fruits.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Models, Molecular
Citrus
Adaptation, Biological
Fruit Crops
Plant Science
01 natural sciences
Biochemistry
Genetically Modified Plants
Xanthomonas citri
RNA interference
Plant Growth Regulators
Gene Expression Regulation, Plant
Gene expression
Genetics
Multidisciplinary
Genetically Modified Organisms
Transcriptional Control
Eukaryota
food and beverages
Agriculture
Plants
Nucleic acids
Protein Transport
Basic-Leucine Zipper Transcription Factors
Phenotype
Genetic interference
Experimental Organism Systems
Citrus canker
Host-Pathogen Interactions
Engineering and Technology
Medicine
Epigenetics
Genetic Engineering
Citrus sinensis
Research Article
Biotechnology
Arabidopsis Thaliana
Science
Bioengineering
Crops
Brassica
Biology
Research and Analysis Methods
Fruits
03 medical and health sciences
Model Organisms
Plant and Algal Models
DNA-binding proteins
Gene silencing
Gene Regulation
Transcription factor
Plant Diseases
Bacterial disease
Organisms
Biology and Life Sciences
Proteins
Biotic stress
Regulatory Proteins
030104 developmental biology
Animal Studies
RNA
Plant Biotechnology
010606 plant biology & botany
Transcription Factors
Crop Science
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 14
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....f01b18a4b18631e26b5140f643701c69