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Rapid Analysis of Bacterial Contamination in Platelets without Pre-Enrichment Using Pig Serum-Derived Antibodies
- Source :
- ACS Applied Bio Materials. 4:7779-7789
- Publication Year :
- 2021
- Publisher :
- American Chemical Society (ACS), 2021.
-
Abstract
- As the shelf life of platelets collected from donated blood is very short, approximately 5 days, the determination of bacterial contamination in platelets has become necessary. In this study, rapid analysis of Gram-positive and Gram-negative bacterial contamination in platelet samples was presented without pre-enrichment using pig serum-derived antibodies against the outer membrane proteins (OMP) of Gram-negative bacteria and antibodies against lipoteichoic acid (LTA) on the surface of Gram-positive bacteria. The anti-OMP antibodies against Gram-negative bacteria were isolated using sequential incubation with (1) the modified Gram-negative bacteria ClearColi, which lacks lipopolysaccharide (LPS) on the outer membrane, and (2) the Gram-positive bacteria
- Subjects :
- Blood Platelets
Immunoassay
biology
Swine
Chemistry
Biochemistry (medical)
Biomedical Engineering
Membrane Proteins
Biosensing Techniques
General Chemistry
Contamination
Gram-Positive Bacteria
Shelf life
Antibodies
Microbiology
Biomaterials
Pre enrichment
Gram-Negative Bacteria
Escherichia coli
biology.protein
Animals
Platelet
Antibody
Subjects
Details
- ISSN :
- 25766422
- Volume :
- 4
- Database :
- OpenAIRE
- Journal :
- ACS Applied Bio Materials
- Accession number :
- edsair.doi.dedup.....f08b9d869bcd99069dbd64d0ae898146