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The Interferon-Inducible MxB Protein Inhibits HIV-1 Infection
- Source :
- Cell Host & Microbe. 14(4):398-410
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- SummaryThe interferon-inducible myxovirus resistance (Mx) proteins play important roles in combating a wide range of virus infections. MxA inhibits many RNA and DNA viruses, whereas the antiviral activity of MxB is less well established. We find that human MxB inhibits HIV-1 infection by reducing the level of integrated viral DNA. Passaging HIV-1 through MxB-expressing cells allowed the evolution of a mutant virus that escapes MxB restriction. HIV-1 escapes MxB restriction by mutating the alanine residue at position 88 in the viral capsid protein (CA), with a consequent loss of CA interaction with the host peptidylprolyl isomerase cyclophilin A (CypA), suggesting a role for CypA in MxB restriction. Consistent with this, MxB associates with CypA, and shRNA-mediated CypA depletion or cyclosporine A treatment resulted in the loss of MxB inhibition of HIV-1. Taken together, we conclude that human MxB protein inhibits HIV-1 DNA integration by a CypA-dependent mechanism.
- Subjects :
- Peptidylprolyl isomerase
0303 health sciences
Cancer Research
biology
030302 biochemistry & molecular biology
Mutant
RNA
Cypa
biology.organism_classification
Microbiology
Virology
Virus
3. Good health
03 medical and health sciences
Cyclophilin A
Capsid
Interferon
Immunology and Microbiology(all)
medicine
Parasitology
Molecular Biology
030304 developmental biology
medicine.drug
Subjects
Details
- ISSN :
- 19313128
- Volume :
- 14
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Cell Host & Microbe
- Accession number :
- edsair.doi.dedup.....f0a0bc94725687e947bdc8a37015ad0c
- Full Text :
- https://doi.org/10.1016/j.chom.2013.08.015