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Comparison of Mid-Turbinate and Nasopharyngeal Specimens for Molecular Detection of SARS-CoV-2 Among Symptomatic Outpatients at a Pediatric Drive-Through Testing Site
- Source :
- Journal of the Pediatric Infectious Diseases Society. 10(8)
- Publication Year :
- 2021
-
Abstract
- Background Nasopharyngeal (NP) specimen testing by reverse transcriptase polymerase chain reaction (RT-PCR) is the standard of care for detecting SARS-CoV-2. Data comparing the sensitivity and specificity of the NP specimen to the less invasive, mid-turbinate (MT) nasal specimen in children are limited. Methods Paired clinical NP and research MT specimens were collected from children Results Out of 907 children, 569 (62.7%) had parental consent and child assent when appropriate to participate and provided paired MT and NP specimens a median of 4 days after symptom onset (range 1-14 days). 16.5% (n = 94) of MT specimens were positive for SARS-CoV-2 compared with 20.0% (n = 114) of NP specimens. The sensitivity of research MT compared to clinical NP specimens was 82.5% (95% CI: 74.2%, 88.9%), specificity was 100.0% (95% CI: 99.2%, 100.0%), and overall agreement was 96.1% (κ = 0.87). The sensitivity of MT specimens decreased with time from 100% (95% CI: 59.0%, 100.0%) on day 1 of illness to 82.1% (95% CI: 73.8%, 88.7%) within 14 days of illness onset; sensitivity was generally >90% when specimens were collected within the first week of illness. Conclusion MT specimens, particularly those collected within the first week of illness, have moderately reduced sensitivity and equivalent specificity to less-tolerated NP specimens in pediatric outpatients. MT specimen use in children may represent a viable alternative to NP specimen collection.
- Subjects :
- medicine.medical_specialty
2019-20 coronavirus outbreak
Standard of care
Coronavirus disease 2019 (COVID-19)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Less invasive
Turbinates
Gastroenterology
03 medical and health sciences
0302 clinical medicine
Internal medicine
Outpatients
medicine
Humans
030212 general & internal medicine
Symptom onset
Child
Nose
0303 health sciences
030306 microbiology
business.industry
SARS-CoV-2
COVID-19
General Medicine
Infectious Diseases
medicine.anatomical_structure
Specimen collection
Pediatrics, Perinatology and Child Health
RNA, Viral
business
Subjects
Details
- ISSN :
- 20487207
- Volume :
- 10
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Journal of the Pediatric Infectious Diseases Society
- Accession number :
- edsair.doi.dedup.....f0a8ba4706022cd66f210b6e4cf75e15