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Qualitative and Quantitative Analysis of the Major Constituents in Chinese Medical Preparation Lianhua-Qingwen Capsule by UPLC-DAD-QTOF-MS
- Source :
- The Scientific World Journal, The Scientific World Journal, Vol 2015 (2015)
- Publication Year :
- 2015
- Publisher :
- Hindawi Limited, 2015.
-
Abstract
- Lianhua-Qingwen capsule (LQC) is a commonly used Chinese medical preparation to treat viral influenza and especially played a very important role in the fight against severe acute respiratory syndrome (SARS) in 2002-2003 in China. In this paper, a rapid ultraperformance liquid chromatography coupled with diode-array detector and quadrupole time-of-flight mass spectrometry (UPLC-DAD-QTOF-MS) method was established for qualitative and quantitative analysis of the major constituents of LQC. A total of 61 compounds including flavonoids, phenylpropanoids, anthraquinones, triterpenoids, iridoids, and other types of compounds were unambiguously or tentatively identified by comparing the retention times and accurate mass measurement with reference compounds or literature data. Among them, twelve representative compounds were further quantified as chemical markers in quantitative analysis, including salidroside, chlorogenic acid, forsythoside E, cryptochlorogenic acid, amygdalin, sweroside, hyperin, rutin, forsythoside A, phillyrin, rhein, and glycyrrhizic acid. The UPLC-DAD method was evaluated with linearity, limit of detection (LOD), limit of quantification (LOQ), precision, stability, repeatability, and recovery tests. The results showed that the developed quantitative method was linear, sensitive, and precise for the quality control of LQC.
- Subjects :
- Article Subject
lcsh:Medicine
Anthraquinones
Capsules
lcsh:Technology
High-performance liquid chromatography
Mass Spectrometry
General Biochemistry, Genetics and Molecular Biology
chemistry.chemical_compound
Rutin
Chlorogenic acid
Medicine
Iridoids
lcsh:Science
Chromatography, High Pressure Liquid
General Environmental Science
Detection limit
Phillyrin
Traditional medicine
lcsh:T
business.industry
Amygdalin
lcsh:R
Salidroside
General Medicine
chemistry
lcsh:Q
business
Quantitative analysis (chemistry)
Research Article
Drugs, Chinese Herbal
Subjects
Details
- ISSN :
- 1537744X and 23566140
- Volume :
- 2015
- Database :
- OpenAIRE
- Journal :
- The Scientific World Journal
- Accession number :
- edsair.doi.dedup.....f0be6edff49aaf24485cf5c052a1ded7
- Full Text :
- https://doi.org/10.1155/2015/731765