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Novel Treatment of Experimental Autoimmune Prostatitis by Nanoparticle-Conjugated Autoantigen Peptide T2
- Source :
- Inflammation. 42(3)
- Publication Year :
- 2019
-
Abstract
- The exact etiology and pathogenesis of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) are still unknown, as a result, available therapeutic options for patients are far from satisfactory. Therefore, there is a need to develop a valid therapeutic approach that can ameliorate the manifestations of CP/CPPS. Fifty male C57BL/6 mice were randomly divided into five groups of ten mice each. All groups except naive were subcutaneously injected with 0.2 ml of T2 plus complete Freund adjuvant (CFA) on day 0 and 14 to generate valid CP/CPPS model. After successful CP/CPPS induction, model group was injected with 0.2 ml of normal saline while PLGA, PLGA-OVA, and PLGA-T2 groups were administered intravenously with 0.2 ml mixture of PLGA, PLGA-OVA, and PLGA-T2, respectively. Voiding behavior, pain threshold, and hematoxylin and eosin staining were used to assess micturition habits, pain intensity as well as prostate inflammation. Additionally, TNF-α, CRP, and IL-10 levels in plasma were measured by using ELISA kits. Mice administered with PLGA-T2 showed higher pain threshold, lower urine frequencies, mild edema, and inflammation in prostate tissue in comparison to other groups. Moreover, the expression of TNF-α and CRP levels was markedly decreased while IL-10 expression was increased in the PLGA-T2 treatment group as compared to the other groups. Our results showed that nanoparticles conjugated with autoantigen novel peptide T2 could successfully alleviate or even heal CP/CPPS to some extent in mice. This study provides an easy, useful, and economic tool for ameliorating the manifestations of CP/CPPS that will improve the therapeutic approaches.
- Subjects :
- 0301 basic medicine
Male
medicine.medical_specialty
Immunology
H&E stain
CD2 Antigens
Prostatitis
Gastroenterology
Autoantigens
Autoimmune Diseases
Pathogenesis
03 medical and health sciences
Mice
0302 clinical medicine
Prostate
Internal medicine
Edema
Threshold of pain
Immunology and Allergy
Medicine
Animals
Autoimmune disease
business.industry
Tumor Necrosis Factor-alpha
medicine.disease
Rheumatology
Interleukin-10
Mice, Inbred C57BL
Disease Models, Animal
030104 developmental biology
medicine.anatomical_structure
C-Reactive Protein
030220 oncology & carcinogenesis
Nanoparticles
medicine.symptom
business
Subjects
Details
- ISSN :
- 15732576
- Volume :
- 42
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Inflammation
- Accession number :
- edsair.doi.dedup.....f0d47790e2011fb5fca3d1d76e142e63