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Whole-Brain Imaging with Single-Cell Resolution Using Chemical Cocktails and Computational Analysis
- Source :
- Cell. 157:726-739
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- SummarySystems-level identification and analysis of cellular circuits in the brain will require the development of whole-brain imaging with single-cell resolution. To this end, we performed comprehensive chemical screening to develop a whole-brain clearing and imaging method, termed CUBIC (clear, unobstructed brain imaging cocktails and computational analysis). CUBIC is a simple and efficient method involving the immersion of brain samples in chemical mixtures containing aminoalcohols, which enables rapid whole-brain imaging with single-photon excitation microscopy. CUBIC is applicable to multicolor imaging of fluorescent proteins or immunostained samples in adult brains and is scalable from a primate brain to subcellular structures. We also developed a whole-brain cell-nuclear counterstaining protocol and a computational image analysis pipeline that, together with CUBIC reagents, enable the visualization and quantification of neural activities induced by environmental stimulation. CUBIC enables time-course expression profiling of whole adult brains with single-cell resolution.
- Subjects :
- Microscopy
Tissue clearing
Biochemistry, Genetics and Molecular Biology(all)
Brain
Callithrix
Neuroimaging
Biology
Bioinformatics
General Biochemistry, Genetics and Molecular Biology
Chemical screening
Mice
Chemical mixtures
Optical clearing
Animals
Indicators and Reagents
Computational analysis
Biomedical engineering
Subjects
Details
- ISSN :
- 00928674
- Volume :
- 157
- Database :
- OpenAIRE
- Journal :
- Cell
- Accession number :
- edsair.doi.dedup.....f0f16bceb1b9dd58457e540db1e2a4ae