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Using mRNA deep sequencing to analyze differentially expressed genes during Panax notoginseng saponin treatment of ischemic stroke
- Source :
- Molecular Medicine Reports
- Publication Year :
- 2020
- Publisher :
- Spandidos Publications, 2020.
-
Abstract
- Treatment with Panax notoginseng saponin (PNS) can prevent neurological damage in middle cerebral artery occlusion model rats to promote recovery after a stroke. However, the exact molecular mechanisms are unknown and require further study. In the present study, mRNA sequencing was employed to investigate differential gene expression between model and sham groups, and between model and PNS‑treated groups. Enrichment of gene data was performed using Gene Ontology analysis and the Kyoto Encyclopedia of Genes and Genomes database. Hub genes were identified and networks were constructed using Cytoscape that were further verified by reverse transcription‑quantitative PCR. A total of 1,104 genes of interest were found, which included 690 upregulated and 414 downregulated genes that were identified when the model was compared with the sham group. Additionally, 817 genes of interest, which included 390 upregulated and 427 downregulated genes, were identified when the PNS‑treated group was compared with the model group. There were 303 overlapping genes of interest between the analysis of model to sham groups, and the analysis of model to PNS‑treated groups. The top 10 genes from the 303 aberrantly expressed genes of interest included ubiquitin conjugating enzyme E2 variant 2, small ubiquitin‑related modifier 1, small RNA binding exonuclease protection factor La, Finkel‑Biskis‑Reilly murine sarcoma virus (FBR‑MuSV) ubiquitously expressed, centrosomal protein 290 kDa, DNA‑directed RNA polymerase II subunit K, cullin‑4B, matrin‑3 and vascular endothelial growth factor receptor 2. In conclusion, these genes may be important in the underlying mechanism of PNS treatment in ischemic stroke. Additionally, the present data provided novel insight into the pathogenesis of ischemic stroke.
- Subjects :
- Male
0301 basic medicine
Cancer Research
Small RNA
middle cerebral artery occlusion
Gene Expression
Panax notoginseng
Plant Roots
Biochemistry
Deep sequencing
Brain Ischemia
Rats, Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Gene expression
ischemic stroke
Genetics
Animals
Gene Regulatory Networks
RNA, Messenger
KEGG
mRNA deep sequencing
Molecular Biology
Gene
Messenger RNA
biology
Gene Expression Profiling
Panax notoginseng saponin
High-Throughput Nucleotide Sequencing
Infarction, Middle Cerebral Artery
Articles
Saponins
biology.organism_classification
Molecular biology
Rats
Stroke
Gene Ontology
030104 developmental biology
MRNA Sequencing
Oncology
030220 oncology & carcinogenesis
Molecular Medicine
Transcriptome
Drugs, Chinese Herbal
Subjects
Details
- ISSN :
- 17913004 and 17912997
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Molecular Medicine Reports
- Accession number :
- edsair.doi.dedup.....f0f7d81180c19e08754d1aee2585aedc