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Axonal Sprouting into the Denervated Spinal Cord and Synaptic and Postsynaptic Protein Expression in the Spinal Cord after Transplantation of Bone Marrow Stromal Cell in Stroke Rats
- Publication Year :
- 2007
-
Abstract
- We investigated whether compensatory reinnervation in the corticospinal tract (CST) and the corticorubral tract (CRT) is enhanced by the administration of bone marrow stromal cells (BMSCs) after experimental stroke. Adult male Wistar rats were subjected to permanent right middle cerebral artery occlusion (MCAo). Phosphate-buffered saline (PBS, control, n=7) or 3x10(6) BMSCs in PBS (n=8) were injected into a tail vein at 1 day postischemia. The CST of the left sensorimotor cortices was labeled with DiI 2 days prior to MCAo. Functional recovery was measured. Rats were sacrificed at 28 days after MCAo. The brain and spinal cord were removed and processed for vibratome sections for laser-scanning confocal analysis and paraffin sections for immunohistochemistry. Normal rats (n=4) exhibited a predominantly unilateral pattern of innervation of CST and CRT axons. After stroke, bilateral innervation occurred through axonal sprouting of the uninjured CRT and CST. Administration of BMSCs significantly increased the axonal restructuring on the de-afferented red nucleus and the denervated spinal motoneurons (p0.05). BMSC treatment also significantly increased synaptic proteins in the denervated motoneurons. These results were highly correlated with improved functional outcome after stroke (r0.81, p0.01). We conclude that the transplantation of BMSCs enhances axonal sprouting and rewiring into the denervated spinal cord which may facilitate functional recovery after focal cerebral ischemia.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Red nucleus
Central nervous system
Article
Central nervous system disease
medicine
Animals
cardiovascular diseases
Rats, Wistar
Molecular Biology
Bone Marrow Transplantation
Denervation
Microscopy, Confocal
business.industry
General Neuroscience
Infarction, Middle Cerebral Artery
Anatomy
Recovery of Function
medicine.disease
Spinal cord
Immunohistochemistry
Axons
Nerve Regeneration
Rats
Transplantation
Stroke
medicine.anatomical_structure
Spinal Cord
Corticospinal tract
Neurology (clinical)
Stromal Cells
business
Developmental Biology
Reinnervation
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....f14c386f426b5c1a0d544658066cfb81