Back to Search
Start Over
In vivo treatment with the herbal phenylethanoid acteoside ameliorates intestinal inflammation in dextran sulphate sodium-induced colitis
- Source :
- Clinical and Experimental Immunology. 148:373-381
- Publication Year :
- 2007
- Publisher :
- Oxford University Press (OUP), 2007.
-
Abstract
- Summary Recently we demonstrated that in inflammatory bowel disease (IBD) macrophage-oxidative burst activity is increased and NADPH oxidase mRNA is induced. The herbal phenylethanoid acteoside isolated from Plantago lanceolata L. was shown to exhibit anti-oxidative potential. Using the dextran sulphate sodium (DSS)-induced colitis model, in this study we have assessed whether systemic application of acteoside affects colitis. Colitis was induced by DSS in Balb/c mice. Treatment with acteoside (120, 600 µg/mouse/day) was performed intraperitoneally. The colon lengths were determined. Colonic tissue was scored histologically (max. score 8) by a blinded investigator. T cells isolated from mesenteric lymph nodes (MLN) were stimulated with anti-CD3 antibody in the presence of interleukin (IL)-2 (final concentration 10 U/ml). After incubation for 24 h, IL-1β, IL-6, IL-12 tumour necrosis factor (TNF)-α and interferon (IFN)-γ levels in supernatants were analysed by the beadlyte® cytokine detection system. Histological scoring of colonic tissue revealed that application of acteoside was followed by a significantly improved histological score. In acute colitis the histological score was 3·2 with acteoside versus 5·2 with phosphate-buffered saline (PBS) (P
- Subjects :
- Necrosis
Colon
medicine.medical_treatment
Immunology
Enzyme-Linked Immunosorbent Assay
Inflammatory bowel disease
Antioxidants
Mice
Glucosides
Phenols
Weight Loss
medicine
Animals
Immunology and Allergy
Mesenteric lymph nodes
Intestinal Mucosa
Colitis
Acute colitis
Peroxidase
Respiratory Burst
Mice, Inbred BALB C
business.industry
Macrophages
Dextran Sulfate
Interleukin
medicine.disease
Respiratory burst
Cytokine
medicine.anatomical_structure
Acute Disease
Chronic Disease
Animal Studies
Cytokines
Female
Lymph Nodes
Inflammation Mediators
medicine.symptom
business
Subjects
Details
- ISSN :
- 13652249 and 00099104
- Volume :
- 148
- Database :
- OpenAIRE
- Journal :
- Clinical and Experimental Immunology
- Accession number :
- edsair.doi.dedup.....f1536db96dc34e913a19a3208ce57fc0