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Catalytic inactivation of influenza virus by iron oxide nanozyme
- Source :
- Theranostics
- Publication Year :
- 2019
- Publisher :
- Ivyspring International Publisher, 2019.
-
Abstract
- Influenza poses a severe threat to human health in the world. However, developing a universal anti-viral strategy has remained challenging due to the presence of diverse subtypes as well as its high mutation rate, resulting in antigenic shift and drift. Here we developed an antiviral strategy using iron oxide nanozymes (IONzymes) to target the lipid envelope of the influenza virus. Methods: We evaluated the antiviral activities of our IONzymes using a hemagglutination assay, together with a 50% tissue culture infectious doses (TCID50) method. Lipid peroxidation of the viral envelope was analyzed using a maleic dialdehyde (MDA) assay and transmission electron microscopy (TEM). The neighboring viral proteins were detected by western blotting. Results: We show that IONzymes induce envelope lipid peroxidation and destroy the integrity of neighboring proteins, including hemagglutinin, neuraminidase, and matrix protein 1, causing the inactivation of influenza A viruses (IAVs). Furthermore, we show that our IONzymes possess a broad-spectrum antiviral activity on 12 subtypes of IAVs (H1~H12). Lastly, we demonstrate that applying IONzymes to a facemask improves the ability of virus protection against 3 important subtypes that pose a threat to human, including H1N1, H5N1, and H7N9 subtype. Conclusion: Together, our results clearly demonstrate that IONzymes can catalyze lipid peroxidation of the viral lipid envelope to inactivate enveloped viruses and provide protection from viral transmission and infection.
- Subjects :
- 0301 basic medicine
Medicine (miscellaneous)
Hemagglutinin (influenza)
02 engineering and technology
medicine.disease_cause
Antiviral Agents
Ferric Compounds
Virus
influenza virus
03 medical and health sciences
Membrane Lipids
Viral envelope
Influenza, Human
medicine
Animals
Humans
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
Peroxidase
antivirus
Mice, Inbred BALB C
Hemagglutination assay
Viral matrix protein
lipoxidase-like activity
biology
Chemistry
Antigenic shift
lipid peroxidation
021001 nanoscience & nanotechnology
Virology
Influenza A virus subtype H5N1
030104 developmental biology
Influenza A virus
biology.protein
Biocatalysis
Female
0210 nano-technology
Neuraminidase
Research Paper
Iron oxide nanozyme
Subjects
Details
- Language :
- English
- ISSN :
- 18387640
- Volume :
- 9
- Issue :
- 23
- Database :
- OpenAIRE
- Journal :
- Theranostics
- Accession number :
- edsair.doi.dedup.....f16e0c39163322df7f9017f41582a847