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Acute Hypoxia and Chronic Ischemia Induce Differential Total Changes in Placental Epigenetic Modifications
- Source :
- Reprod Sci
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Preeclampsia is a common obstetrical complication, hallmarked by new-onset hypertension. Believed to result from placental insufficiency and chronic placental ischemia, the symptoms of preeclampsia are caused by release of pathogenic factors from the placenta itself, although the mechanisms of their regulation are in many cases unknown. One potential mechanism is through changes in placental epigenetic chromatin modifications, particularly histone acetylation and DNA methylation. Here, we determined the effects of chronic ischemia on global epigenetic modifications in the rodent placenta in vivo and acute hypoxia in BeWo placental trophoblast cells in vitro. Placental insufficiency via uterine artery restriction increased maternal blood pressure and fetal demise while decreasing placental and fetal mass. Global placental histone H3 acetylation levels were significantly decreased at H3 K9, K14, K18, K27, and K56. Interestingly, when BeWo-immortalized placental trophoblast cells were cultured in oxygen concentrations mimicking healthy and ischemic placentas, there was a significant increase in acetylated at K9, K18, K27, and K56. This was associated with a small but significant decrease in placental acetyl-CoA, suggesting depletion in the source of acetyl group donors. Finally, while global methylation of cytosine from placental DNA was low in both groups of animals (
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Placenta
Ischemia
Blood Pressure
Placental insufficiency
Cell Line
Epigenesis, Genetic
Preeclampsia
Histones
Rats, Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Pre-Eclampsia
Acetyl Coenzyme A
Pregnancy
Internal medicine
medicine
Animals
Aorta, Abdominal
Epigenetics
Hypoxia
Histone H3 acetylation
reproductive and urinary physiology
030219 obstetrics & reproductive medicine
biology
business.industry
Ovary
Obstetrics and Gynecology
Trophoblast
Acetylation
Arteries
Original Articles
DNA Methylation
Surgical Instruments
medicine.disease
Rats
Trophoblasts
Disease Models, Animal
030104 developmental biology
Endocrinology
medicine.anatomical_structure
Histone
embryonic structures
biology.protein
Female
business
Subjects
Details
- ISSN :
- 19337205 and 19337191
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Reproductive Sciences
- Accession number :
- edsair.doi.dedup.....f185e84568216add0b2e83ca5ad06ad3